Engineering Endochondral Bone: In Vivo Studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Serafim M. Oliveira (Autor) FEUP; Dindo Q. Mijares (Autor) Outra; Gloria Turner (Autor) Outra; Isabel F. Amaral (Autor) FEUP; Mário A. Barbosa (Autor) FEUP; Cristina C. Teixeira (Autor) Outra

Revista

Título: Tissue Engineering - Part A

Vol. 15 Nº 3

Páginas: 635-643

ISSN: 1937-3341

Editora: Mary Ann Liebert

Indexação

ISI Web of Knowledge - 28 Citações

ISI Web of Science

Scopus - 34 Citações

COMPENDEX

Classificação Científica

FOS: Ciências médicas e da saúde > Biotecnologia médica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-003-N3D

DOI: 10.1089/ten.tea.2008.0052

Abstract (EN): The use of biomaterials to replace lost bone has been a common practice for decades. More recently, the demands for bone repair and regeneration have pushed research into the use of cultured cells and growth factors in association with these materials. Here we report a novel approach to engineer new bone using a transient cartilage scaffold to induce endochondral ossification. Chondrocyte/chitosan scaffolds (both a transient cartilage scaffold-experimental-and a permanent cartilage scaffold-control) were prepared and implanted subcutaneously in nude mice. Bone formation was evaluated over a period of 5 months. Mineralization was assessed by Faxitron, micro computed tomography, backscatter electrons, and Fourier transform infrared spectroscopy analyses. Histological analysis provided further information on tissue changes in and around the implanted scaffolds. The deposition of ectopic bone was detected in the surface of the experimental implants as early as 1 month after implantation. After 3 months, bone trabeculae and bone marrow cavities were formed inside the scaffolds. The bone deposited was similar to the bone of the mice vertebra. Interestingly, no bone formation was observed in control implants. In conclusion, an engineered transient cartilage template carries all the signals necessary to induce endochondral bone formation in vivo.

Idioma: Inglês

Tipo (Avaliação Docente): Científica