A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid's proteome

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Trindade, F (Autor) Outra; Bastos, P (Autor) Outra; Leite-Moreira AF (Autor) FMUP; Manadas, B (Autor) Outra; Ferreira, R (Autor) Outra; Soares, SF (Autor) Outra; Daniel da Silva, AL (Autor) Outra; Falcao Pires, I (Autor) FMUP; Vitorino, R (Autor) FMUP

Revista

Título: Archives of Biochemistry and Biophysics

Vol. 634

Páginas: 1-10

ISSN: 0003-9861

Editora: Elsevier

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00N-2H5

DOI: 10.1016/j.abb.2017.09.016

Abstract (EN): Owing to their close proximity, pericardial fluid (PF)'s proteome may mirror the pathophysiological status of the heart. Despite this diagnosis potential, the knowledge of PF's proteome is scarce. Large amounts of albumin hamper the characterization of the least abundant proteins in PR. Aiming to expand PF's proteome and to validate the technique for future applications, we have fractionated and characterized the PF, using N-(trimethoxysilylpropyl)ethylenediamine triacetic acid (EDTA)-functionalized magnetic nanoparticles (NPs@EDTA) followed by a GeLC-MS/MS approach. Similarly to an albumin depletion kit, NPs@EDTA-based fractionation was efficient in removing albumin. Both methods displayed comparable inter-individual variability, but NPs@EDTA outperformed the former with regard to the protein dynamic range as well as to the monitoring of biological processes. Overall, 565 proteins were identified, of which 297 (>50%) have never been assigned to PR. Moreover, owing to this method's good proteome reproducibility, affordability, rapid automation and high binding ability of NP@EDTA, it bears a great potential towards future clinical application.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10