Simple Determination of Deoxycholic and Ursodeoxycholic Acids by Phenolphthalein-beta-Cyclodextrin Inclusion Complex

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Pabyton G Cadena (Autor) Outra; Eric C Oliveira (Autor) Outra; Alberto N Araujo (Autor) FFUP; Maria C B S M Montenegro (Autor) FFUP; Maria C B Pimentel (Autor) Outra; Jose L Lima Filho (Autor) Outra; Valdinete L Silva (Autor) Outra

Revista

Título: Lipids

Vol. 44

Páginas: 1063-1070

ISSN: 0024-4201

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 7 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Ciências da saúde

Outras Informações

ID Authenticus: P-003-ESX

DOI: 10.1007/s11745-009-3353-z

Abstract (EN): An expeditious colorimetric methodology for the determination of the deoxycholic acid (DCA) and of the ursodeoxycholic acid (UDCA) in pharmaceutical formulations is reported. The method is based on their competitive complexation reaction with a color indicator to form beta-cyclodextrin-inclusion complexes. Several pH color indicators were tested, but phenolphthalein (PHP) showed the best interaction with the beta-cyclodextrin (beta-CD) with an inclusion yield higher than 95%. The best concentrations of beta-cyclodextrin to form inclusion complexes were 1.24 x 10(-3) and 6.2 x 10(-4) M at pH 9.5 and 10.5. Statistical analysis of the results showed that the pH had a significant effect on the DCA determination and that high beta-CD-PHP inclusion complex concentrations had a significant negative effect on the UDCA determination (p < 0.05). The limit of detection and limit of quantification were 3.94 x 10(-5) and 1.31 x 10(-4) M for DCA (range: 6.1 x 10(-6)-3.13 x 10(-3) M), 4.08 x 10(-5) and 1.36 x 10(-4) M for UDCA (range: 6.05 x 10(-6)-3.88 x 10(-4) M). This simple and cheap method showed high stability and feasible instrumentation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8