Automatic quantification of cell outgrowth from neurospheres

• Type: Article in International Conference Proceedings Book
• Date: 2013

Authors

Bessa, S (Author) Other; Quelhas, P (Author) Other; Amaral, IF (Author) FEUP

Conference proceedings International

Title: Pattern Recognition and Image Analysis - 6th Iberian Conference, IbPRIA 2013, Funchal, Madeira, Portugal, June 5-7, 2013. Proceedings

Pages: 141-148

6th Iberian Conference on Pattern Recognition and Image Analysis, IbPRIA 2013

Funchal, Madeira, 5 June 2013 through 7 June 2013

Indexing

Scopus - 0 Citations

Other information

Authenticus ID: P-008-DEJ

DOI: 10.1007/978-3-642-38628-2_16

Abstract (EN): In the development of new regenerative medicine therapies for the treatment of central nervous system and spinal cord injuries, the identification of factors that inhibit or promote cell outgrowth in neurite outgrowth assays is fundamental, and the neurotrophic activity is commonly assessed based on the neurite/cell outgrowth. Neurites are projections from the cell body or the initial neurosphere and typically present low-contrast to background in phase contrast images. The extent of neurites is usually measured in a manual way and fluorescence images are the most used, generally requiring imunofluorescent staining. We present a novel automatic approach for the quantification of cell outgrowth from neurospheres, based on phase contrast and fluorescence images acquired from samples merely processed for DNA staining. Our approach detects the neurite/cell outgrowth, and its measures are in high agreement with the ones obtained manually. Furthermore, the image analysis time was reduced in more than 95% allowing the increase of the amount of data to be analyzed. © 2013 Springer-Verlag.

Language: English

Type (Professor's evaluation): Scientific