Projectos de Doutoramento (PHD Projects) II

Divulgação

PhD project is available Title: Evolutionary sorting of large DNA segments in cancer and genomic disorders Duration of thesis: The expected duration of the thesis is about 4 years. Admission Requirements: - Candidates should hold an academic qualification “licenciatura” (Bachelor of Science) degree in: Biology, Biochemistry, Medicine; Veterinary Medicine or in related fields. - Experience in Molecular and/or Cellular Biology or in areas related with the project is required. - Candidates should have MS degree in an area related with the project or at least 16 points according to the Portuguese classification system. Working Place: The project will involve international exchange and cooperation. Karolinska Institute, Microbiology and Tumorbiology Center Stockholm, Sweden, and Centre of Human Genetics, National Institute of Health “Dr. Ricardo Jorge”, Av. Padre Cruz, 1649-016 Lisbon, Portugal. Supervisors: Stefan Imreh, PhD Associate Professor, Karolinska Institute Microbiology and Tumorbiology Center Nobelsväg 16 17177 Stockholm, Sweden tel: 08-52486770 mobile: 073-9065595 fax: 08-330498 e-mail: Stefan.Imreh@mtc.ki.se Dezsö David Ph.D., Associate Researcher, Centre of Human Genetics National Institute of Health “Dr. Ricardo Jorge” Av. Padre Cruz 1649-016 Lisboa Portugal Fax: (+351) 217526410 Telephone: (+351) 217519322 e-mail: dezso.david@insa.min-saude.pt Financial Support and Conditions: Please note that funding needs to be obtained either by the selected applicant alone, or a joint application involving the candidate and the supervisor must submited for the scholarships available at Fundação para a Ciência e a Tecnologia (FCT) http://www.fct.mces.pt/pt/apoios/bolsas/concursosabertos/. This application contains three parts: one regarding the Ph.D. student, one regarding the supervisor and one including the project proposal. Details of the scholarships are also available at the same site (please follow link). The amount of the scholarship (in Portugal is 980¤/month and abroad 1.7550¤/month). Applications, Deadlines and Evaluation of Candidates: Applications can be submitted either to Stockholm or to Lissabon in Portuguese or English and most include: a cover letter, detailed CV (including summaries of previous works or publications) at least two recommendation letters (including contact informations) and academic transcript. Deadline for the applications is 5 March 2006. Deadline for the project proposal is 31 of March. According to FCT successful students will initiate their project in January 2007. For further information please contact one of the Supervisors. Candidates will be evaluated and pre-selected based on the submitted documents. Only the pre-selected group of candidates will be informed and invited for personal interview or contacted regarding the final selection process. Description of the project: The project intends to explain the apparent contradiction between the relatively large size of cancer associated deletions and the presumption that these are linked to inactivation of individual genes. We intend to test functionally the following hypothesis: Genes and regulatory units in the genome are sorted during the evolution on a functional basis. Conserved segments that are stable both in evolution and functionally, may contain multiple genes/regulatory units that control normal mitotic activity. In contrast cancer associated breakpoint regions coincide with synteny breaks, contain multiple paralogs or segmental duplications and other instability features (including miRNA sites) and are called evolutionarily plastic regions. Evolutionary plasticity may be involved in genomic disorders including constitutional translocations. The evolutionary stable and plastic regions have different expression pattern. The project trains in 1. tumor biology, 2. human genetics, 3. comparative genomics, 4. bioinformatics and will include experiments concerning chromosome transfer, transfer of large 100-800 kb genomic fragments in normal regulatory context, RNAi inactivation of key genes and serial inactivation of multiple genes in candidate regions. Along molecular methods FISH based cytogenetics will be employed. Please consult the group recent papers i.e.: Kost-Alimova M et al, Proc Natl Acad Sci USA, 100, 6622-6627, 2003, Imreh S et al, Genes Chromosomes and Cancer, 38, 307-321, 2003, Sharp TV et al, Proc Natl Acad Sci USA,101, 16531-15636, 2004, Darai E et al, Genomics,86, 1-12, 2005. Kutsche K et al, Nature Genetics, 26: 247-250, 2000. David D et al, Genomics, 81: 489-503, 2003.