Production and Characterization of Graphene Oxide Surfaces against Uropathogens

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Belo, S (Autor) Outra; Sousa-Cardoso, F (Autor) Outra; Rita Teixeira dos Santos (Autor) FEUP; Luciana Gomes (Autor) FEUP; Vieira, R (Autor) Outra; Sjollema, J (Autor) Outra; Soares, OSGP (Autor) Outra; Filipe Mergulhão (Autor) FEUP

Revista

Título: Coatings

Vol. 13

Página Final: 1324

ISSN: 2079-6412

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Projetos Associados

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00Y-T54

DOI: 10.3390/coatings13081324

Abstract (EN): Graphene and its functionalized derivatives have been increasingly applied in the biomedi-cal field, particularly in the production of antimicrobial and anti-adhesive surfaces. This study aimed to evaluate the performance of graphene oxide (GO)/polydimethylsiloxane (PDMS) composites against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. GO/PDMS composites containing different GO loadings (1, 3, and 5 wt.%) were synthesized and characterized regarding their morphol-ogy, roughness, and hydrophobicity, and tested for their ability to inhibit biofilm formation under conditions that mimic urinary tract environments. Biofilm formation was assessed by determining the number of total and culturable cells. Additionally, the antibacterial mechanisms of action of GO were investigated for the tested uropathogens. Results indicated that the surfaces containing GO had greater roughness and increased hydrophobicity than PDMS. Biofilm analysis showed that the 1 wt.% GO/PDMS composite was the most effective in reducing S. aureus biofilm formation. In oppo-sition, P. aeruginosa biofilms were not inhibited by any of the synthesized composites. Furthermore, 1% (w/v) GO increased the membrane permeability, metabolic activity, and endogenous reactive oxygen species (ROS) synthesis in S. aureus. Altogether, these results suggest that GO/PDMS com-posites are promising materials for application in urinary catheters, although further investigation is required.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17