Sulfate-based lipids: Analysis of healthy human fluids and cell extracts

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Dias, IHK (Autor) Outra; Ferreira, R (Autor) Outra; Gruber, F (Autor) Outra; Vitorino, R (Autor) FMUP; Rivas Urbina, A (Autor) Outra; Sanchez Quesada, JL (Autor) Outra; Silva, JV (Autor) Outra; Fardilha, M (Autor) Outra; Victor de Freitas (Autor) FCUP; Reis, A (Autor) Outra

Revista

Título: Chemistry and Physics of Lipids

Vol. 221

Páginas: 53-64

ISSN: 0009-3084

Editora: Elsevier

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-00Q-DTC

DOI: 10.1016/j.chemphyslip.2019.03.009

Abstract (EN): Sulfate-based lipids (SL) have been proposed as players in inflammation, immunity and infection. In spite of the many biochemical processes linked to SL, analysis on this class of lipids has only focused on specific SL subclasses in individual fluids or cells leaving a range of additional SL in other biological samples unaccounted for. This study describes the mass spectrometry screening of SL in lipid extracts of human fluids (saliva, plasma, urine, seminal fluid) and primary human cells (RBC, neutrophils, fibroblasts and skin epidermal) using targeted precursor ion scanning (PIS) approach. The PIS 97 mass spectra reveal a wide diversity of SL including steroid sulfates, sulfoglycolipids and other unidentified SL, as well as metabolites such as taurines, sulfated polyphenols and hypurate conjugates. Semi-quantification of SL revealed that plasma exhibited the highest content of SL whereas seminal fluid and epithelial cells contained the highest sulphur to phosphorous (S/P) ratio. The complexity of biofluids and cells sulfateome presented in this study highlight the importance of expanding the panel of synthetic sulfate-based lipid standards. Also, the heterogenous distribution of SL provides evidence for the interplay of sulfotransferases/sulfatases, opening new avenues for biomarker discovery in oral health, cardiovascular, fertility and dermatology research areas.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12