Expression pattern of G protein-coupled receptor 30 in human seminiferous tubular cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Oliveira, PF (Autor) Outra; Alves, MG (Autor) Outra; Martins, AD (Autor) Outra; Correia, S (Autor) Outra; Bernardino, RL (Autor) Outra; Silva, J (Autor) Outra; barros, a (Autor) FMUP; sousa, m (Autor) ICBAS; Cavaco, JE (Autor) Outra; Socorro, S (Autor) Outra

Revista

Título: General and Comparative Endocrinology

Vol. 201

Páginas: 16-20

ISSN: 0016-6480

Editora: Elsevier

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 21 Citações

Outras Informações

ID Authenticus: P-009-BYW

DOI: 10.1016/j.ygcen.2014.02.022

Abstract (EN): The role of estrogens in male reproductive physiology has been intensively studied over the last few years. Yet, the involvement of their specific receptors has long been a matter of debate. The selective testicular expression of the classic nuclear estrogen receptors (ER alpha and ER beta) argues in favor of ER-specific functions in the spermatogenic event. Recently, the existence of a G protein-coupled estrogen receptor (GPR30) mediating non-genomic effects of estrogens has also been described. However, little is known about the specific testicular expression pattern of GPR30, as well as on its participation in the control of male reproductive function. Herein, by means of immunohistochemical and molecular biology techniques (RT-PCR and Western blot), we aimed to present the first exhaustive evaluation of GPR30 expression in non-neoplastic human testicular cells. Indeed, we were able to demonstrate that GPR30 was expressed in human testicular tissue and that the staining pattern was consistent with its cytoplasmic localization. Additionally, by using cultured human Sertoli cells (SCs) and isolated haploid and diploid germ cells fractions, we confirmed that GPR30 is expressed in SCs and diploid germ cells but not in haploid germ cells. This specific expression pattern suggests a role for GPR30 in spermatogenesis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5