Sequential dual site-selective protein labelling enabled by lysine modification

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Matos, MJ (Author) FCUP; Brown, L (Author) Other; Bernardim, B (Author) Other; Guerreiro, A (Author) Other; Jimenez Oses, G (Author) Other; Bernardes, GJL (Author) Other

Journal

Title: Bioorganic and Medicinal Chemistry

Vol. 28

ISSN: 0968-0896

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00S-RSW

DOI: 10.1016/j.bmc.2020.115783

Abstract (EN): Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6