Interaction of natural compounds with biomembrane models: A biophysical approach for the Alzheimer's disease therapy

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Stephanie Andrade (Autor) FEUP; Maria J. Ramalho (Autor) FEUP; Joana A. Loureiro (Autor) FEUP; Maria do Carmo Pereira (Autor) FEUP

Revista

Título: Colloids and Surfaces B: Biointerfaces

Vol. 180

Páginas: 83-92

ISSN: 0927-7765

Editora: Elsevier

Indexação

ISI Web of Knowledge - 2 Citações

ISI Web of Science

Scopus - 14 Citações

INSPEC

Projetos Associados

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00Q-QFC

DOI: 10.1016/j.colsurfb.2019.04.019

Abstract (EN): Natural compounds such as caffeine (CA), gallic acid (GA) and tannic acid (TA) have been reported to be useful for Alzheimer's disease (AD) therapy. It was proved that some natural compounds inhibit the formation of senil plaques composed by beta-amyloid peptide (A beta), a hallmark of AD. Evidences suggest that the therapeutic activity of compounds depends of their interaction with biological membranes. To understand why these compounds fail in vivo and in clinical trials, it is important to evaluate their pharmacokinetics properties. Thus, a biophysical approach to study drug-membrane interactions is essential to understand the mechanisms by which the drugs interact with the cellular membranes and affect the A beta production, aggregation and clearance pathways. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and cholesterol (chol) were used to mimic the biophysical properties of cell membranes and study their interactions with these compounds. The partition coefficient, influence on membrane fluidity and location within the bilayer of the drugs were studied by derivative spectrophotometry, dynamic light scattering and fluorescence quenching, respectively.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10