Uncertainty in the determination of glucose in aqueous solutions by high-performance liquid chromatography with evaporative light scattering detection

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Berta Nogueiro Estevinho (Author) FEUP; Amélia Ferraz (Author) Other; Fernando Rocha (Author) FEUP; Lúcia Santos (Author) FEUP; Arminda Alves (Author) FEUP

Journal

Title: Journal of Separation Science

Vol. 32 No. 4

Pages: 3116-3125

ISSN: 1615-9306

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 10 Citations

ISI Web of Science

Scopus - 12 Citations

COMPENDEX

Scientific classification

CORDIS: Technological sciences > Engineering > Chemical engineering

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-G9N

DOI: 10.1002/jssc.200900205

Abstract (EN): The determination of glucose and other carbohydrates is the most widespread chemical analysis that is performed within the industries of food, beverage, forage, biomass, pull) and paper, pharmaceuticals among others. Besides that, sugar refineries need to control their products, by-products and effluents, and furthermore, glucose in the sucrose refining process, is considered an impurity, which shall be controlled. Being HPLC the most currently instrumental technique used for glucose analysis, the evaporative light scattering detector (ELSD) offers advantages (sensitivity, possibility for operating in gradient mode) over the also used refractive index detector. In this work, an HPLC-ELSD methodology was optimised and validated, aiming the estimate of the uncertainty associated with the results at low levels of concentration of glucose to be measured. Linearity of the response was obtained in the range of glucose concentrations from 20 to 300 mg/L, with an analysis time of 10 min. The global uncertainty was estimated accordingly to the bottom-up approach used by Eurachem. It was 13% on average for concentrations from 100 to 300 mg/L. For lower concentrations, uncertainty increased significantly up to 30% in the vicinity of the LOD of the method.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10