FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Sílvia Fontenete (Author) Other; Carvalho, D (Author) Other; Lourenco, A (Author) Other; Guimaraes, N (Author) FEUP; Madureira, P (Author) Other; Figueiredo C (Author) FMUP; Azevedo, NF (Author) FEUP

Journal

Title: Biochemical Engineering Journal

Vol. 112

Pages: 61-69

ISSN: 1369-703X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 12 Citations

INSPEC

Associated Projects

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

DNA mimics - Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00K-CJ9

DOI: 10.1016/j.bej.2016.04.001

Abstract (EN): Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determination of fluorescent intensity in microscopy images still has issues due to the lack of precision in the results and the complexity of existing software. This work presents FISHji, a set of new ImageJ methods for automated quantification of fluorescence in images obtained by epifluorescence microscopy. To validate the methods, results obtained by FISHji were compared with results obtained by flow cytometry. The mean correlation between FISHji and flow cytometry was high and significant, showing that the imaging methods are able to accurately assess the signal intensity of fluorescence images. FISHji are available for non-commercial use at http://paginas.fe.up.pt/similar to nazevedo/.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9