Validation of a high performance liquid chromatography method for the stabilization of epigallocatechin gallate

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Fangueiro, JF (Author) Other; Parra, A (Author) Other; Silva, AM (Author) Other; Egea, MA (Author) Other; Souto, EB (Author) Other; Garcia, ML (Author) Other; Calpena, AC (Author) Other

Journal

Title: International Journal of Pharmaceutics

Vol. 475

Pages: 181-190

ISSN: 0378-5173

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-009-ST5

DOI: 10.1016/j.ijpharm.2014.08.053

Abstract (EN): Epigallocatechin gallate (EGCG) is a green tea catechin with potential health benefits, such as anti-oxidant, anti-carcinogenic and anti-inflammatory effects. In general, EGCG is highly susceptible to degradation, therefore presenting stability problems. The present paper was focused on the study of EGCG stability in HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) medium regarding the pH dependency, storage temperature and in the presence of ascorbic acid a reducing agent. The evaluation of EGCG in HEPES buffer has demonstrated that this molecule is not able of maintaining its physicochemical properties and potential beneficial effects, since it is partially or completely degraded, depending on the EGCG concentration. The storage temperature of EGCG most suitable to maintain its structure was shown to be the lower values (4 or 20 degrees C). The pH 3.5 was able to provide greater stability than pH 7.4. However, the presence of a reducing agent (i.e., ascorbic acid) was shown to provide greater protection against degradation of EGCG. A validation method based on RP-HPLC with UV-vis detection was carried out for two media: water and a biocompatible physiological medium composed of Transcutol (R) P, ethanol and ascorbic acid. The quantification of EGCG for purposes, using pure EGCG, requires a validated HPLC method which could be possible to apply in pharmacokinetic and pharmacodynamics studies. (C) 2014 Published by Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10