Saltar para:
Logótipo
Comuta visibilidade da coluna esquerda
Você está em: Início > Publicações > Visualização > Detection of Legionella spp. in natural and man-made water systems using standard guidelines

Detection of Legionella spp. in natural and man-made water systems using standard guidelines

Título
Detection of Legionella spp. in natural and man-made water systems using standard guidelines
Tipo
Artigo em Revista Científica Internacional
Ano
2012
Autores
Anabela Borges
(Autor)
FEUP
Manuel José Simões
(Autor)
FEUP
Antonia Martínes-Murcia
(Autor)
Outra
A pessoa não pertence à instituição A pessoa não pertence à instituição A pessoa não pertence à instituição Sem AUTHENTICUS Sem ORCID
Maria José Saavedra
(Autor)
Outra
A pessoa não pertence à instituição A pessoa não pertence à instituição A pessoa não pertence à instituição Sem AUTHENTICUS Sem ORCID
Revista
Indexação
Publicação em ISI Web of Science ISI Web of Science
Classificação Científica
FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental
Outras Informações
ID Authenticus: P-00M-GGX
Abstract: Infections caused by Legionella spp. are considered at the present time, an emerging public health problem and are linked to high rates of mortality and morbidity, if not properly treated. In this study were analyzed 54 samples of water from 8 counties at Northern Portugal, with the aim of obtaining a collection of strains of the genus Legionella and to characterize them genetically and phenotypically. Another objective of this study was to evaluate the effectiveness of the technique of cultivation, a standard method according to International Organization for Standardization ISO 11731:1998, for detection and enumeration of species of Legionella. For laboratory processing, after the filtration of samples (1 L), the filtrate was resuspended in sterile distilled water (5 ml). Heat treatment for selective inhibition of non-Legionella bacteria was performed. Subsequently, 100 ¿l of the suspension was spread in GVPC selective agar medium, and incubated (7 to 10 days) at 37 ¿. Colonies that were morphologically characteristic of the genus were sub-cultured onto BCYE agar and blood agar for verification. According to the procedure recommended by the standard method, only the colonies which grew in BCYE agar and not on blood agar were considered as suspected Legionella strains. The identification of these initially selected colonies was performed by sequencing the 16S rRNA gene, which revealed that none of the isolates were identified as belonging to the genus Legionella. However, through the ISO 11731:1998 they were interpreted as positive, corresponding therefore to ¿false-positive¿ results. The methods used in this study allowed the isolation of a number of isolates (40), which form an independent group of all genus of the family Chitinophagaceae outlined so far, and that by their phylogenetic distance might be a genus not yet described and therefore a new species. The results obtained, highlighted the importance of using culture and genetic methods in parallel for the proper identification of microorganisms
Idioma: Inglês
Tipo (Avaliação Docente): Científica
Nº de páginas: 8
Documentos
Não foi encontrado nenhum documento associado à publicação com acesso permitido.
Publicações Relacionadas

Das mesmas áreas científicas

Injectable alginate hydrogels for cell delivery in tissue engineering (2014)
Outra Publicação em Revista Científica Internacional
Silvia J Bidarra; Cristina C Barrias; Pedro L Granja
Functionalization of biomaterials with small osteoinductive moieties (2013)
Outra Publicação em Revista Científica Internacional
Raquel Maia, FR; Silvia J Bidarra; Pedro L Granja; Cristina C Barrias

Ver todas (311)

Da mesma revista

Action of kanamycin against single and dual species biofilms of Escherichia coli and Staphylococcus aureus (2012)
Artigo em Revista Científica Internacional
Ana Margarida Pereira; Ana Cristina Abreu; Manuel Simões
Recomendar Página Voltar ao Topo
Copyright 1996-2019 © Reitoria da Universidade do Porto  I Termos e Condições  I Acessibilidade  I Índice A-Z  I Livro de Visitas
Página gerada em: 2019-04-22 às 19:44:12 | Política de Utilização Aceitável | Política de Proteção de Dados Pessoais