Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Vera Faustino (Autor) Outra; Diana Pinho (Autor) Outra; Tomoko Yaginuma (Autor) Outra; Ricardo C. Calhelha (Autor) Outra; Isabel C. F. R. Ferreira (Autor) Outra; Rui Lima (Autor) FEUP

Revista

Título: Biochip Journal

Vol. 8 Nº 1

Páginas: 42-47

ISSN: 1976-0280

Editora: Springer Nature

Indexação

ISI Web of Science

Scopus

INSPEC

Projetos Associados

ANEURYSM - ANEURYSM - Estudo Hemodinâmico em Aneurismas Cerebrais

Outras Informações

DOI: 10.1007/s13206-014-8107-1

Abstract (EN): Red blood cell (RBC) deformability has become one of the important factors to assess blood and cardiovascular diseases. The interest on blood studies have promoted a development of various microfluidic devices that treat and analyse blood cells. Recent years, besides the RBC deformability assessment, these devices are often applied to cancer cell detection and isolation from the whole blood. The devices for cancer cell isolation rely mainly on size and deformability of the cells. However, the examination of deformability of the RBCs mixed with cancer cells is lacking. This study aims at determining the deformation index (DI) of the RBCs in contact with cancer cells using a hyperbolic microchannel which generates a strong extensional flow. The DIs of human healthy RBCs and human RBCs in contact with a tumor cell line (HCT-15, colon carcinoma) were compared by analyzing the flowing RBCs images captured by a high speed camera. The results reveal that the RBCs that were in contact with HCT-15 cells have lower deformability than the normal RBCs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6