Protein Crystallization As a Process Step in a Novel Meso Oscillatory Flow Reactor: Study of Lysozyme Phase Behavior

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Filipa Castro (Autor) FEUP; António Ferreira (Autor) FEUP; José A. Teixeira (Autor) Outra; Fernando Rocha (Autor) FEUP

Revista

Título: Crystal Growth & Design

Vol. 16 Nº 7

Páginas: 3748-3755

ISSN: 1528-7483

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 8 Citações

ISI Web of Science

Scopus - 16 Citações

Classificação Científica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

FOS: Ciências da engenharia e tecnologias > Engenharia química

Projetos Associados

IF/01087/2014/CP1249/CT0001 - Projeto de investigação exploratória

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

Outras Informações

ID Authenticus: P-00K-P3Q

DOI: 10.1021/acs.cgd.6b00262

Abstract (EN): In the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 min. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8