Design and characterization of Nanostructured lipid carriers (NLC) and Nanostructured lipid carrier-based hydrogels containing Passiflora edulis seeds oil

• Type: Article in International Scientific Journal
• Date: 2021-03-10

Authors

Karolline Krambeck (Author) FFUP; Maria Helena Amaral (Author) FFUP

Journal

Title: International Journal of Pharmaceutics

Vol. 600

ISSN: 0378-5173

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 40 Citations

Other information

Authenticus ID: P-00T-NC3

DOI: 10.1016/j.ijpharm.2021.120444

Abstract (EN): This study aims to design and characterize Nanostructured lipid carriers (NLC) and Nanostructured lipid carrier-based hydrogels with Passiflora edulis seeds oil, a by-product from Madeira Island food industry. NLC were prepared by the ultrasonication technique, using passion fruit seeds oil as a liquid lipid and glyceryl distearate as a solid lipid. These NLC were then gelled with Poly (acrylic acid). Long-term stability studies were conducted with NLC and NLC-based hydrogels stored for 12 months. The following tests were performed: morphology, encapsulation efficiency, particle size analysis, polydispersity index analysis, zeta potential, pH measurement, color analysis, viscosity studies, texture analysis, in vitro occlusion test, ex vivo skin penetration study, tyrosinase inhibition activity, in vitro skin permeation experiments and in vitro cytotoxicity studies. The developed NLC had spherical shape and narrow particle sizes distribution with mean sizes in the range of 150 nm and PDI below 0.3, Zeta potential values around -30 mV and high Encapsulation efficiency. The tyrosinase inhibitory activity and skin retention of the nanoparticles was superior to that of the non-encapsulated oil. The developed formulations did not show cytotoxicity towards HaCat cells and presented suitable viscosity and texture properties for skin application, proving to be good candidates as depigmenting agent.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11