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Engineering and Molecular Biology of Plants

Code: M.BIO031     Acronym: EBMP

Keywords
Classification Keyword
OFICIAL Molecular Bioengineering

Instance: 2023/2024 - 2S (of 05-02-2024 to 24-05-2024) Ícone do Moodle

Active? Yes
Responsible unit: Molecular Biology
Course/CS Responsible: Master in Bioengineering

Cycles of Study/Courses

Acronym No. of Students Study Plan Curricular Years Credits UCN Credits ECTS Contact hours Total Time
M.BIO 15 Syllabus 1 - 6 39 162

Teaching language

Portuguese

Objectives

 

This curricular unit is intended to provide students basic training necessary for the understanding and application of bioengeneering in plants. Students should become fully aware of the vital importance of plants in providing food, water, medicines, the air we breathe, wood, paper, habitats, climate and many other essential resources and of the potential of plants as a tool of excellence for bioengineering.

Students should acquire a solid grounding in the fundamental concepts of plant biology and its wide range of applications in biotechnology. Students should gain an in-depth knowledge of the molecular processes underlying plant physiology and morphology, and of the molecular methodologies available to study these processes and its use in biotechnology. 
Students are expected to acquire skills to use modern plant bioengineering strategies and tools for practical applications such as developing nutritionally enhanced foods, enabling sustainable agricultural production, developing plants capable of copping with climate changes and engineering plants for industrial and medical purposes.

 

Learning outcomes and competences

Students should acquire skills to:


1) Interpret and understand the molecular processes underlying plant physiology


2) Research and develop specific knowledge in plant biology, knowing how to employ the available tools


3) Perform a critical analysis of the experimental results and their interpretation in light of acquired theoretical knowledge


4) Formulate experimental strategies involving molecular methods to study the functional biology of plants and


5) Know how to use current plant bioengineering tools and strategies to solve problems in the field of plant biotechnology.

 

Working method

Presencial

Pre-requirements (prior knowledge) and co-requirements (common knowledge)

Molecular and Cell Biology

Program

1 – The plant systems. Plant cell, body plan, organs and tissues; photosynthesis, respiration and photorespiration; metabolic flow in plants- primary and secondary metabolism; plant development - morphogenesis, hormones and signal transduction, plant genetics- specificities and models.


2- Molecular methodologies used in plant biotechnology. Transgenic plants – methods of transformation, regeneration and applications;; modulation of gene expression- knockouts, silencing, over–expression, alteration of the gene expression products; plant genome editing using the CRISPR/cas system;  plant functional genomics- genomes, transcriptomics, proteomics, metabolomics and biocomputing tools.


3- Plants for the future. Plant improvement (nutritional content, productivity, tolerance to stress, pathogen and herbicides, phytoremediation); plants as production systems (molecular farming) - plants as green factories for the production of vaccines, pharmaceuticals, plastics, biomaterials, and biofuels.

Mandatory literature

Buchanan, Gruissem and Jones; Biochemistry and Molecular Biology of Plants, Buchanan, Gruissem and Jones, 2000. ISBN: 0-943088-39-9
Lincoln Taiz; Plant physiology. ISBN: 978-0-87893-856-8

Complementary Bibliography

Carlos Azevedo, Claudio Sunkel; Biologia Molecular e Celular- 5.ª edição, Lidel, 2012. ISBN: 978-972-757-692-0
Arie Altman, Paul Michael Hasegawa; Plant Biotechnology and Agriculture- Prospects for the 21st century, Academic Press, 2012. ISBN: 978-0-12-381466-1
Adrian Slater, Nigel W Scott and MarK R Fowler; Plant Biotechnology-The genetic manipulation of plants, Adrian Slater, Nigel W Scott and MarK R Fowler, 2008. ISBN: 978-0-19-928261-6

Teaching methods and learning activities

The central themes in Plant Biology are presented by the lecturer, followed by an in-depth discussion involving the presentation of relevant scientific articles  by the students. 
At the end of the semester, students must select a specific question related to the topics covered in class and present a research project on plant bioengineering. The project should include the objective, an introduction in which the literature on the chosen topic is reviewed, a work plan describing the experimental strategy and methods to be used, the expected results and their contribution to the advancement of knowledge. This bioengineering project aims to encourage students to apply the theoretical knowledge they have learned to a practical case, promoting critical thinking and problem-solving skills in a real context.

 

keywords

Natural sciences > Biological sciences > Botany > Plant physiology
Natural sciences > Biological sciences > Biological engineering > Genetic engineering
Natural sciences > Biological sciences > Biology > Molecular biology
Technological sciences > Technology > Biotecnology > Plant biotechnology

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation Weight (%)
Defesa pública de dissertação, de relatório de projeto ou estágio, ou de tese 40,00
Exame 40,00
Apresentação/discussão de um trabalho científico 20,00
Total: 100,00

Amount of time allocated to each course unit

Designation Time (hours)
Apresentação/discussão de um trabalho científico 2,00
Elaboração de projeto 6,00
Frequência das aulas 39,00
Total: 47,00

Eligibility for exams

Attendance at 75% of classes. Completion of all components of the evaluarion process.

Calculation formula of final grade

Distributed evaluation with final exam.

Final classification (NF) = (NT x 0.4) + (0.4 x PI) + (0.2 x AA)

where

NT, exam classification mark

PI, classification mark of the research project

AA average of the marks obtained for the presentation of scientific papers

To pass the course, the final grade can not be lower than 9.5 and the exam classification mark can not be lower than 9,0.

Internship work/project

At the end of the semester, students should present a research project on plant bioengineering. Students must select a specific problem related to the topics covered in class that can be solved through plant bioengineering. The project should include the following components: the aim of the project; an introduction reviewing the literature on the chosen topic; a work plan describing the experimental strategy and methods to be used; expected results and their contribution to solving the problem in question and advancing knowledge in the chosen topic.

Classification improvement

It will be possible to improve one or more components of the evaluation within the legally defined conditions and deadlines.
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