Induction and subcellular redistribution of progesterone receptor A and B by tamoxifen in the hypothalamic ventromedial neurons of young adult female Wistar rats

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Sa, SI (Autor) FMUP; Fonseca, BM (Autor) FFUP; Teixeira, NA (Autor) FFUP; Madeira, MD (Autor) FMUP

Revista

Título: Molecular and Cellular Endocrinology

Vol. 420

Páginas: 1-10

ISSN: 0303-7207

Editora: Elsevier

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-00K-0YE

DOI: 10.1016/j.mce.2015.11.015

Abstract (EN): The ventrolateral division of the hypothalamic ventromedial nucleus (VMNvl) is a brain center for estrogen-dependent triggering of female sexual behavior upon progesterone receptor (PR) activation. We examined the agonistic and antagonistic actions of tamoxifen in this nucleus by analyzing its effects on the total number of PR-immunoreactive neurons, PR mRNA and protein levels, and subcellular location of PRs in ovariectomized Wistar rats. The results show that tamoxifen has no agonistic action in the number of PR-immunoreactive neurons, but increases PR expression and labeling in the nucleus and cytoplasm of VMNvl neurons that constitutively express PRs. As an antagonist, tamoxifen partially inhibited the estradiol-dependent increase in the number of PR-immunoreactive neurons and in PR mRNA and protein levels, without interfering with the subcellular location of the protein. We suggest that tamoxifen influence on PR expression in the VMNvl critically depends on the presence or absence of estradiol.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10