Use of response surface methodology to optimize protease synthesis by a novel strain of Bacillus sp isolated from Portuguese sheep wool

• Type: Article in International Scientific Journal
• Date: 2012

Authors

A. C Queiroga (Author) Other; M. E. Pintado (Author) Other; F. X. Malcata (Author) FEUP

Journal

Title: JOURNAL OF APPLIED MICROBIOLOGY

Vol. 113

Pages: 36-43

ISSN: 1364-5072

Indexing

ISI Web of Knowledge - 9 Citations

ISI Web of Science

Scopus - 14 Citations

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Other information

Authenticus ID: P-002-8NE

DOI: 10.1111/j.1365-2672.2012.05300.x

Abstract (EN): Aims: To investigate the influence of yeast extract, peptone, temperature and pH upon protease productivity by Bacillus sp. HTS102 a novel wild strain isolated from wool of a Portuguese sheep breed (Merino). Methods and Results: A 24 full factorial, central composite design together with response surface methodology was used to carry out the experiments and analyse the results, respectively. Among the individual parameters tested, temperature and peptone concentration produced significant effects upon protease productivity. A high correlation coefficient (R2 = 0.994, P < 0.01) indicated that the empiric second-order polynomial model postulated was adequate to predict said productivity, with the optimum loci characterized by: temperature of 43 degrees C, peptone content of 1.4 g l-1, pH of 5.1 and yeast extract concentration of 10.0 g l-1. Conclusions: Protease synthesis depends chiefly on temperature and peptone level. The maximum protease activity was more than twice that obtained with the basal medium, so the experimental design and analysis undertaken were effective towards process optimization. Significance and Impact of the Study: Rational choice of processing conditions for maximum protease productivity will be relevant if an economically feasible fermentation process based on Bacillus sp. HTS102 is intended.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8