Single determination of alpha-ketoglutaric acid and pyruvic acid in beer by HPLC with UV detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Patricia Montenegro (Autor) Outra; Ines Maria Valente (Autor) Outra; Luis Moreira Goncalves (Autor) Outra; Jose Antonio Rodrigues (Autor) FCUP; Aquiles Araujo Barros (Autor) FCUP

Revista

Título: Analytical Methods

Vol. 3

Páginas: 1207-1212

ISSN: 1759-9660

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 21 Citações

Scopus - 23 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-002-RQB

DOI: 10.1039/c0ay00669f

Abstract (EN): The present work describes a simple and straightforward methodology for the determination of alpha-ketoglutaric acid (KA) and pyruvic acid (PA) in beer. This work involves three basic steps: (a) cleaning of the sample by solid phase extraction (SPE); (b) alpha-ketoacids' derivatization with o-phenylenediamine (OPDA) to form quinoxalines; and (c) separation and detection of the quinoxalines by high-performance liquid chromatography with spectrophotometric detection (HPLC-UV). The identity of the eluted compounds was also confirmed by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in the positive ion mode. The developed methodology showed good repeatability (ca. 4%) and linearity as well as good limits of detection (0.091 and 0.055 mg L(-1) for KA and PA, respectively) and quantification (0.30 and 0.18 mg L(-1) for KA and PA, respectively). To sum up, the goal of this technical note is to develop a single HPLC procedure with previous SPE clean up suitable to use in routine analysis laboratories.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6