Autonomous Optical Tweezers: From automatic trapping to single particle analysis

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2022

Autores

Coutinho, F (Autor) Outra; Teixeira, J (Autor) Outra; Rocha, V (Autor) Outra; Oliveira, J (Autor) Outra; Jorge, PAS (Autor) FCUP; Silva, NA (Autor) Outra

Ata de Conferência Internacional

Título: Journal of Physics: Conference Series

5th International Conference on Applications of Optics and Photonics, AOP 2022

17 July 2022 through 22 July 2022

Indexação

Scopus

Outras Informações

ID Authenticus: P-00X-Q7Y

DOI: 10.1088/1742-6596/2407/1/012025

Abstract (EN): Optical trapping is a versatile and non-invasive technique for single particle manipulation. As such, it can be widely applied in the domains of particle identification and classification and thus used as a tool for monitoring physical and chemical processes. This creates an opportunity for integrating the method seamlessly into optofluidic chips, provided it can be automatized. Yet even though OT is well established in multiple scientific domains, a full stack approach to its integration into other technological devices is still lacking. This calls for solutions in tasks such as automatic trapping and signal analysis. In this manuscript, we describe the implementation of an algorithm seeking autonomous particle location and trapping. The methodology is based upon image-processing, allowing for particle location using real time image segmentation. A local thresholding algorithm is applied, followed by morphological techniques for closing shapes and excluding non-bounded regions - after which only the particles remain on the image. Once the centroid is identified, the stage is translated accordingly by piezo-electric actuators, followed by the laser activation. In this way, trapping is achieved, and one may proceed to analyze the forward scattered optical signal, after which a new particle inside the actuators range may be automatically trapped. This development, when compared with existent solutions involving holographic optical tweezers, allows for similar capabilities without using a spatial light modulator, thus dramatically reducing the setup costs of autonomous OT solutions. Therefore, when combined with particle classification techniques, this method is well suited for integration into possible optofluidic chips for autonomous sensing and monitoring of biochemical samples. © Published under licence by IOP Publishing Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica