Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2021

Autores

Teresa Cardoso (Autor) ICBAS; Almeida, AS (Autor) Outra; Fernando Remiao (Autor) FFUP; Carla Fernandes (Autor) FFUP

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: Chemosensors

Vol. 34

Página Final: 304

ISSN: 2227-9040

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-00V-PKF

DOI: 10.3390/chemosensors9110304

Abstract (EN): The interaction between proteins and drugs or other bioactive compounds has been widely explored over the past years. Several methods for analysis of this phenomenon have been developed and improved. Nowadays, increasing attention is paid to innovative methods, such as high performance affinity liquid chromatography (HPALC) and affinity capillary electrophoresis (ACE), taking into account various advantages. Moreover, the development of separation methods for the analysis and resolution of chiral drugs has been an area of ongoing interest in analytical and medicinal chemistry research. In addition to bioaffinity binding studies, both HPALC and ACE al-low one to perform other type of analyses, namely, displacement studies and enantioseparation of racemic or enantiomeric mixtures. Actually, proteins used as chiral selectors in chromatographic and electrophoretic methods have unique enantioselective properties demonstrating suitability for the enantioseparation of a large variety of chiral drugs or other bioactive compounds. This review is mainly focused in chromatographic and electrophoretic methods using human serum albumin (HSA), the most abundant plasma protein, as chiral selector for binding affinity analysis and enantioresolution of drugs. For both analytical purposes, updated examples are presented to highlight recent applications and current trends.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 34