Tolerance to arsenic contaminant among multidrug-resistant and copper-tolerant Salmonella successful clones is associated with diverse ars operons and genetic contexts

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Mourao, J (Autor) Outra; Rebelo, A (Autor) Outra; Ribeiro, S (Autor) Outra; Peixe, L. (Autor) FFUP; Novais, Carla (Autor) FFUP; Antunes, Patrícia (Autor) FCNAUP

Revista

Título: Environmental Microbiology

Vol. 22

Páginas: 2829-2842

ISSN: 1462-2912

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 15 Citações

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências médicas e da saúde

Projetos Associados

FOODnanoHEALTH - FOODnanoHEALTH

Outras Informações

ID Authenticus: P-00S-1WG

DOI: 10.1111/1462-2920.15016

Abstract (EN): Emergence and expansion of frequent multidrug-resistant (MDR) major Salmonella clones/serotypes has been a significant threat in the last years. Metal compounds, such as copper, commonly used in animal-production settings, have been pointed out as possible contributors for the selection of such strains/clones. However, the scarcity of studies limits our understanding of the impact of other metal environmental contaminants as arsenic (used in insecticides/herbicides/coccidiostats). We analysed arsenic tolerance (AsT) dispersion by phenotypic and genotypic (PCR/sequencing/I-CeuI/S1/XbaI-PFGE/hybridization) assays among Salmonella with diverse epidemiological and genetic backgrounds. Then, to better understand ars operon genetic contexts, the whole genome of five representative strains was sequenced. We found a high dispersion of ars operons conferring AsT, especially among copper-tolerant and relevant serotypes/clones related to pig-production setting. The acr3-type was found dispersed in the chromosome of diverse serotypes, including the emergent S. Rissen. Conversely, arsBII was almost confined to the MDR ST34 European clone of S. Typhimurium/S. 4,[5],12:i:-, always along with copper/silver tolerance sil + pco clusters in an integrative conjugative element. These data suggest that AsT is an essential adaptive feature for the ecological success of these epidemic clones/serotypes and alerts for global strategies to reduce arsenic-based compounds' impact thus preventing environmental/food contamination with frequent MDR foodborne pathogens.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14