Microrheological characterisation of Cyanoflan in human blood plasma

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024-02

Autores

Campo Deano, L (Autor) FEUP; Rodrigues, T. (Autor) FEUP; Mota, R. (Autor) Outra; Gales, L. (Autor) Outra; Tamagnini, P (Autor) Outra

Revista

Título: Carbohydrate Polymers

Vol. 326

ISSN: 0144-8617

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-HEV

DOI: 10.1016/j.carbpol.2023.121575

Abstract (EN): Naturally occurring polysaccharidic biopolymers released by marine cyanobacteria are of great interest for numerous biomedical applications, such as wound healing and drug delivery. Such polymers generally exhibit high molecular weight and an entangled structure that impact the rheology of biological fluids. However, biocompatibility tests focus not so much on rheological properties as on immune response. In the present study, the rheological behaviour of native blood plasma as a function of the concentration of a cyanobacterium biopolymer is investigated via multiple particle tracking microrheology, which measures the Brownian motion of probes embedded in a sample, and cryogenic scanning electron microscope microstructural characterisation. We use Cyanoflan as the biopolymer of choice, and profit from our knowledge of its chemical structure and its exciting potential for biotechnological applications. A sol-gel transition is identified using time-concentration superposition and the power-law behaviour of the incipient network's viscoelastic response is observed in a variety of microrheological data. Our results point to rheology-based principles for blood compatibility tests by facilitating the assignment of quantitative values to specific properties, as opposed to more heuristic approaches.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12