Involvement of linear plasmids in aerobic biodegradation of vinyl chloride

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Anthony Steven Danko (Autor) FEUP; Meizhong Luo (Autor) Outra; Christopher E. Bagwell (Autor) Outra; Robin L. Brigmon (Autor) Outra; David L. Freedman (Autor) Outra

Revista

Título: Applied and Environmental Microbiology

Vol. 70 Nº 10

Páginas: 6092-6097

ISSN: 0099-2240

Editora: American Society for Microbiology

Indexação

ISI Web of Science

Scopus

COMPENDEX

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Naturais > Ciências biológicas > Biologia > Microbiologia

Outras Informações

DOI: 10.1128/aem.70.10.6092-6097.2004

Abstract (EN): Pseudomonas putida strain AJ and Ochrobactrum strain TD were isolated from hazardous waste sites based on their ability to use vinyl chloride (VC) as the sole source of carbon and energy under aerobic conditions. Strains AJ and TD also use ethene and ethylene oxide as growth substrates. Strain AJ contained a linear megaplasmid (approximately 260 kb) when grown on VC or ethene, but it contained no circular plasmids. While strain AJ was growing on ethylene oxide, it was observed to contain a 100-kb linear plasmid, and its ability to use VC as a substrate was retained. The linear plasmids in strain AJ were cured, and the ability of strain AJ to consume VC, ethene, and ethylene oxide was lost following growth on a rich substrate (LuriaBertani broth) through at least three transfers. Strain TD contained three linear plasmids, ranging in size from approximately 90 kb to 320 kb, when growing on VC or ethene. As with strain AJ, the linear plasmids in strain TD were cured following growth on Luria-Bertani broth and its ability to consume VC and ethene was lost. Further analysis of these linear plasmids may help reveal the pathway for VC biodegradation in strains AJ and TD and explain why this process occurs at many but not all sites where groundwater is contaminated with chloroethenes. Metabolism of VC and ethene by strains AJ and TD is initiated by an alkene monooxygenase. Their yields during growth on VC (0.15 to 0.20 mg of total suspended solids per mg of VC) are similar to the yields reported for other isolates (i.e., Mycobacterium sp., Nocardioides sp., and Pseudomonas sp.).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6