Instance: 2015/2016 - 2S
Cycles of Study/Courses
Teaching Staff - Responsibilities
Teaching - Hours
Suitable for English-speaking students
Prepare students for the appropriate use of animals in scientific research, through the acquisition of knowledge of the fundamental principles of animal experimentation.
Learning outcomes and competences
It is intended that the student: (1) have knowledge of the legislation regulating the use of experimental animals in Europe and Portugal. (2) Know the potential alternatives to animal experiments. (3) Have an attitude towards the use of animals in research that reflects "The 3 Rs": Replace, Reduce, and Refine - with focus on animal welfare and scientific quality for animal experiments. (4) Know the anatomy, biology and major diseases of laboratory animals. (5) Understand the principles behind anesthesia, analgesia, humane endpoints and euthanasia. (6) Know the potential health hazards related to animal experiments, and how to minimize them. (7) Know how to restrain the animals, collect biological samples, and administrate drugs.
Theoretical course: 1. Introduction to the use of animals in scientific research. 3Rs principle. 2. Legal and ethical aspects of animal experimentation. 3. Biology and husbandry of laboratory animals. 4. Anatomy and post-mortem examination of laboratory animals. 5. Restrain and handling of laboratory animals. 6. Anesthesia and analgesia. 7. Euthanasia methods in animal experimentation. 8. Experimental procedures: 8.1. Harvest of biological samples. 8.2. Drug administration. 9. General principles of surgery and basic concepts about suture. 10. Diseases in Laboratory Animals. 11. Zoonosis and microbiological control of laboratory animals. 12. Alternatives to animal experiments.
Practical course: Restrain and handling of laboratory animals. Collection of biological samples. Drug delivery routes (subcutaneous, intramuscular, intraperitoneal, intravenous). Anesthesia and analgesia. Anatomy and necropsy of laboratory animals. Running multiple sutures techniques.
van Zutphen, L. F., Baumans, V., & Beynen, A. C. ; Principles of laboratory animal science: a contribution to the humane use and care of animals and to the quality of experimental results. , Elsevier Science Publishers., 1993
Harkness, J. E., Wagner, J. E. ; The biology and medicine of rabbits and rodents. , Lippincott Williams & Wilkins., 1995
Fox, J. G., Anderson, L. C., Loew, F. M., & Quimby, F. W. ; Laboratory Animal Medicine, American College of Laboratory Animal Medicine, 2002
Flecknell, P. ; Laboratory animal anaesthesia, Elsevier, 2009
Suckow, M. A., Weisbroth, S. H., & Franklin, C. L. (Eds.). ; The laboratory rat. , Academic Press, 2006
Teaching methods and learning activities
The teaching methodology includes theoretical and practical classes, as follows:
-Lectures with audio-visual resources.
-Practical classes (Restrain methods, collection of biological samples, drug administration routes, induction of anaesthesia, performing suture and necropsy techniques).
Evaluation with final exam
Eligibility for exams
Frequency: presence of at least 75% of classes
Calculation formula of final grade
Type of evaluation: Final written examination (Multiple choice test + written short answers).
The classification is obtained with the final exam.