Using plasma-mediated covalent functionalization of rhamnolipids on polydimethylsiloxane towards the antimicrobial improvement of catheter surfaces

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Dardouri, M (Author) Other; Bettencourt, A (Author) Other; Victor Martin (Author) FMDUP; Carvalho, FA (Author) Other; Santos, C (Author) Other; Monge, N (Author) Other; Santos, NC (Author) Other; Fernandes MH (Author) FMDUP; Gomes PS (Author) FMDUP; Ribeiro, IAC (Author) Other

Journal

Title: Materials Science and Engineering C

Vol. 134

ISSN: 0928-4931

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00V-VNY

DOI: 10.1016/j.msec.2021.112563

Abstract (EN): Controlling bacterial biofilm formation on silicone-based bloodstream catheters is of great concern to prevent related infections. In this study, rhamnolipids (RLs), glycolipid biosurfactants, specifically a RLs mixture and the purified di-RL (RhaRhaC10:0C10:0) were covalently bonded to silicone with the intention of reaching long-lasting antibiofilm surfaces. RLs mixture and di-RL were identified by an UHPLC-MS method that also allowed the confirmation of compound isolation by automated flash chromatography. Silicone surfaces underwent air-plasma treatment, inducing reactive oxygen radicals able to promote the RLs grafting that was confirmed by contact angle, FTIR-ATR and AFM measurements. The antibiofilm activity towards different Gram positive strains was evaluated by colony forming units (CFU) count and confocal laser microscopy. In addition, protein adsorption and biocompatibility were also investigated. RLs were successfully grafted onto silicone and RLs mixture and RhaRhaC10C10:0 functionalized specimens reduced the biofilm formation over 2.3 log units against methicillin sensitive Staphylococcus aureus. Additionally, a decrease of 1 log unit was observed against methicillin resistant S. aureus and S. epidermidis. Functionalized samples showed cytocompatibility towards human dermal fibroblasts, hemocompatibility and no vascular irritation potential. The results mentioned above revealed a synergy between the antimicrobial and the anti-adhesive properties of RLs, making these compounds good candidates for the improvement of the medical devices antibiofilm properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14