Abstract (EN):
Strain DC-200(T) was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. The isolate was a Gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10-37 degrees C, pH 6.0-9.5 and with up to 5% of NaCl. The peptidoglycan was of the type B1 alpha and the muramic acid residues were glycolylated. The major fatty acids were anteiso-C(15:0) and anteiso-C(17:0). The predominant respiratory menaquinones were MK-11 and MK-12. The G + C content of the genomic DNA was 70 mol%. Based on the analysis of the 163 rRNA gene sequence, the closest phylogenetic neighbours of strain DC-200(T) were Microbacterium lacus A5E-52(T) (98.7%) and Microbacterium aoyamense KV-492(T) (98.2%). The phenetic characterization of the isolate supports its inclusion within the genus Microbacterium; however, its distinctive phenotypic features and the results from the 16S rRNA gene sequence analysis and the DNA-DNA hybridization study suggest that the isolate represents a novel species. The name Microbacterium invictum sp. nov. is proposed. The type strain is DC-200(T) (=DSM 19600(T)=LMG 24557(T)).
Language:
English
Type (Professor's evaluation):
Scientific
No. of pages:
6