Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Angela França (Author) Other; Virgínia Carvalhais (Author) Other; Manuel Vilanova (Author) ICBAS; Gerald B. Pier (Author) Other; Nuno Cerca (Author) Other

Journal

Title: AMB Express

Vol. 6

Initial page: 23

ISSN: 2191-0855

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 22 Citations

ISI Web of Science

Scopus - 20 Citations

Pubmed / Medline

Other information

Authenticus ID: P-00K-AET

DOI: 10.1186/s13568-016-0197-9

Abstract (EN): Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and active mechanisms, although the last occurred to a lesser extent. Moreover, it was observed that S. epidermidis biofilm-released cells presented higher tolerance to vancomycin and tetracycline, as well as a particular gene expression phenotype when compared with either biofilm or planktonic cells. Using this model, biofilm-released cells phenotype and their interaction with the host immune system could be studied in more detail, which could help providing significant insights into the pathophysiology of biofilm-related infections.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11