Involvement of carbon dioxide in the aerobic biodegradation of ethylene oxide, ethene, and vinyl chloride

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Anthony Steven Danko (Author) Other; Freedman, DL (Author) Other

Journal

Title: Process Biochemistry

Vol. 43

Pages: 517-521

ISSN: 1359-5113

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-003-ZXW

DOI: 10.1016/j.procbio.2008.01.008

Abstract (EN): The involvement of a carboxylase in metabolism of C-2 alkenes by Ochrobactrum sp. strain TD and Pseudomonas putida strain AJ was examined. With resting cells of strain TD grown on vinyl chloride, ethene, and ethylene oxide, the maximum specific rate of ethylene oxide consumption decreased significantly in the absence of external CO2 in comparison to cells provided with room air or added CO2. The amount of (CO2)-C-14 incorporated into biomass by resting cells of strain TD grown on ethylene oxide increased more than 13-fold when the assay substrate was ethylene oxide versus acetate. These results indicate that strain TD uses a carboxylase. Similar experiments were performed with strain AJ with the results suggesting that a carboxylase is not involved. In this regard, strain AJ is more similar to various Mycobacterium isolates that also do not appear to use a carboxylase during metabolism of vinyl chloride and ethene.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5