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Methods in neuroscience

Code: PDN13_7     Acronym: PDN13_7

Classification Keyword
OFICIAL Neurosciences

Instance: 2020/2021 - SP (of 01-09-2020 to 31-07-2021) Ícone do Moodle

Active? Yes
Responsible unit: Departamento de Biomedicina
Course/CS Responsible: PhD Neurosciences

Cycles of Study/Courses

Acronym No. of Students Study Plan Curricular Years Credits UCN Credits ECTS Contact hours Total Time
PDN 2 Plano Oficial do ano letivo 2019 1 - 6 40 162

Teaching Staff - Responsibilities

Teacher Responsibility
Fani Lourença Moreira Neto

Teaching - Hours

Theoretical and practical : 0,21
Laboratory Practice: 1,21
Type Teacher Classes Hour
Theoretical and practical Totals 6 1,26
Ana Maria Marques Valentim 0,027
Ana Cristina Estrela de Oliveira Charrua Cordeiro 0,054
António Avelino Ferreira Saraiva Silva 0,068
Armando Cardoso 0,004
Clara Maria Pires Costa Bastos Monteiro 0,036
Dora Raquel da Silva Pinho 0,014
Maria Dulce Cordeiro Madeira 0,005
Fani Lourença Moreira Neto 0,07
Manuel Fernando dos Santos Barbosa 0,043
Filipe Almeida Monteiro 0,064
Maria Isabel Torres Martins 0,006
Isaura Ferreira Tavares 0,027
Manuela Sofia Rodrigues Morato 0,007
Miguel Filipe Tavares da Luz Soares 0,107
Nikolay Vsevolodovich Lukoyanov 0,071
Paulo José Campos Linhares Vieira 0,018
Paulo Ricardo Baptista Silva Branco 0,123
Paulo Jorge da Silva Correia de Sá 0,057
Pedro Alberto da Graça Pereira 0,002
Sofia Alexandra Duque Santos Fernandes 0,018
Teresa Maria de Jesus Teixeira de Sousa 0,013
Vasco Miguel Clara Lopes Galhardo 0,079
Joana Maria de Pinho Ferreira Gomes 0,212
Laboratory Practice Totals 6 7,26
Ana Cristina Estrela de Oliveira Charrua Cordeiro 0,179
António Avelino Ferreira Saraiva Silva 0,021
Armando Cardoso 0,011
Boris Safronov 0,007
Carlos Manuel Gomes Reguenga 0,179
Célia da Conceição Duarte Cruz 0,134
Daniel Humberto Pozza 0,188
Dora Raquel da Silva Pinho 0,008
Fani Lourença Moreira Neto 0,134
Maria Isabel Torres Martins 0,025
Maria Mafalda Vieira Pereira Gonçalves de Sousa 0,021
Miguel Filipe Tavares da Luz Soares 0,286
Paulo Jorge da Silva Correia de Sá 0,114
Pedro Alberto da Graça Pereira 0,011
Sofia Alexandra Duque Santos Fernandes 0,179
Teresa Maria de Jesus Teixeira de Sousa 0,008
Helder Rui Cardoso da Cruz 0,036
Joana Maria de Pinho Ferreira Gomes 0,22
Clara Maria Pires Costa Bastos Monteiro 0,14

Teaching language

Suitable for English-speaking students


This UC aims at providing students with theoretical and practical education on various methods employed in Neuroscience, which they will have the opportunity to learn by voluntarily choosing a range of optional modules,  according to their scientific interests and putative PhD thesis.

Learning outcomes and competences

The students are offered a vast range of options distributed by eighteen distinct curricular UC components (modules),
from which they must select six. By being aware of the fundaments and mode of employment of the various techniques, the students develop the ability to correctly plan the experimental approach to various scientific questions within the neuroscience domain. These competences will help him to design a research project, either during his PhD education or after getting his(her) degree, and will allow him to understand and criticize the results of others, a skill that is crucial for the buildup of a solid body of knowledge.

Working method



Module 1 – Pharmacological Approaches - António Albino Teixeira (coordenador)

Module 2 – In vivo microdialysis - Maria Isabel Martins (coordinator)

Module 3 - Microscopic Analysis - António Avelino (coordinator)

Module 4 – Live Cell Imaging - Paulo Correia de Sá (coordinator)

Module 5 – Stereological Methods - Dulce Madeira (coordinator)

Module 6 – Neuronal Culture- Ana Charrua (coordinator)

Module 7 – Production and Validation of Animal Models – Fani Neto (coordinator)

Module 8 - Genetically manipulated animal models- Miguel Soares (coordinator)

Module 9 – Stereotaxic Surgery and Microsurgery -Fani Neto (coordinator)

Module 10 – TractTracing Techniques- Isaura

Tavares (coordinator)

Module 11 – Electrophysiology - Vasco Galhardo (coordinator)

Module 12 – Behavioral Evaluation - Clara Monteiro e Joana Ferreira Gomes (coordinators)

Module 13 – Brain Imaging - Isabel Ramos (coordinator)

Module 14 – Essential Bioinformatics – Filipe Monteiro (coordinator)

Module 15 - Computational Neuroscience - Mafalda Sousa (ccordinator)

Module 16 - Signal processing - Paulo Branco (coordinator)

Module 17 - Biostatistics - Rui Magalhães (coordinator)

Mandatory literature

RD Goldman & DL Specto (eds) .; Live Cell Imaging: A Laboratory Manual, , New York, NY: Cold Spring Harbour Laboratory Press, 2005
R Ian Freshney; Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition, New Jersey, WileyBlackwell, 2010
L. W. Haynes (editor); Neuron in Tissue Culture, IBRO Handbook series: methods in Neuroscience volume 18, New Jersey, John Wiley & Sons. ISBN: 1999
G Paxinos & C Watson (eds); The rat brain in stereotaxic coordinates, 6th Edition, Sydney, Academic press, 2006
L.R. Squire et al., (eds); Fundamental Neuroscience, 3rd Edition, Canada, Academic press, 2008

Complementary Bibliography

S Peterson; Drug microinjection in discrete brain regions.Tujunga, California, Kopf Carrier, 50 16., (www.kopfinstruments.com/Carrier/downloads/Carrier50.pdf, 1998
Schaefer PW et al.,; Diffusionweighted MR imaging of the brain. Radiology 217:33145, 2000

Teaching methods and learning activities

The student can count on the advice of his (her) tutor as to the choice of the contents that better fit them in terms
of previous training and future research. The unit is based on practical, hands on classes in laboratory environment, in small size groups. The small size of the groups is guaranteed, if necessary, by multiplying the practical sessions. A few theoretical lessons will provide the students with the fundaments of the methodologies being addressed. The students have access to appropriate bibliography in advance and are instructed to approach professors at any time they need their support.


Health sciences > Neuroscience > Neurobiology

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Designation Weight (%)
Apresentação/discussão de um trabalho científico 20,00
Participação presencial 20,00
Teste 20,00
Trabalho escrito 20,00
Trabalho laboratorial 20,00
Total: 100,00

Amount of time allocated to each course unit

Designation Time (hours)
Apresentação/discussão de um trabalho científico 12,00
Estudo autónomo 100,00
Frequência das aulas 40,00
Trabalho escrito 10,00
Total: 162,00

Eligibility for exams

The student needs to have frequency in each of the six chosen modules with minimal approval in each; Evaluation takes into account the students' critical capacity and previous preparation for each session, as well as their performance at the lab. Depending of each module it may consist on an individual written test, written essays, oral presentation of scientific research papers, presentation and discussion of the experimental work performed in classes, design and presentation of a small exploratory project and/or student´s performance during classes.

Calculation formula of final grade

The final score is the average of the scores obtained at each of the six modules selected by the student:


Classification improvement

Improvement of classification will be permitted in all modules which possess a final written test to all students. In the remaining modules without a final written test, the calssification might be improved by attending again each module separatelly in a new year ("Melhoria por Frequência").
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