Molecular Biology

Code:

MFC16

Acronym:

BiologMolec

Keywords
Classification	Keyword
OFICIAL	Medicine

Instance: 2015/2016 - 1S

Active?	Yes
Responsible unit:	Physiology and Cardiothoracic Surgery Department
Course/CS Responsible:	Cardiovascular and Respiratory Sciences

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MFC	8	Current Studies Plan	1	-	6	54	162

Teaching language

Suitable for English-speaking students

Objectives

Acquire knowledge and develop basic skills for carrying out scientific projects in the cardiovascular area that require the use of molecular techniques.

Learning outcomes and competences

Students are expect to:

1.Learn and follow good laboratorial practices.

2.Understand the basic principles underlying a proper collection, preservation and processing of biological samples for molecular analysis.

3.Plan and execute at least two techniques of molecular biology, among the following: extraction, quantification and analysis of DNA, extraction, quantification and analysis of mRNA or extraction, quantification and analysis of protein.

4.Develop an experimental protocol using molecular biology techniques.

5.Solve scientific problems.

6.Critically evaluate scientific papers of the area.

7.Analyse and present scientific results arising from the used techniques.

Working method

Presencial

Program

Lectures:

1.Good laboratorial practices.

2.DNA and mRNA quantification and analysis.

3.Protein quantification and analysis.

4.ELISA, Genomic, transcriptomics and proteomics.

 

Practical classes:

1.Primers design.

2.Extraction and quantification of mRNA.

3.Proteins extraction and western blotting.

4.ELISA.

 

E-learning:

1.Critical analysis of results derived from molecular analysis techniques.

Mandatory literature

Sambrook, J., & Russel, D. W. ; Molecular Cloning, a laboratory manual third edition., 2001
Freshney, R. I. ; Culture of specific cell types. , John Wiley & Sons, Inc., 2005
Pasterkamp, G., & de Kleijn, D. (Eds.). ; Cardiovascular research: new technologies, methods, and applications. , Springer Science & Business Media. , 2007

Teaching methods and learning activities

Theoretic-practical: 16 hours (including 2 hours for evaluation)

Other: 14 hours

Laboratory Practices: 24 hours

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation	Weight (%)
Exame	50,00
Trabalho escrito	25,00
Trabalho laboratorial	25,00
Total:	100,00

Calculation formula of final grade

Final evaluation will be based on:

1. 25%: continuous assessment based on students-teachers interaction during the hours of contact in particular laboratorial classes.
2. 50%: classification obtained in a final written exam comprising 20 multiple choice or fast-answer questions.
3. 25%: E-learning module whose evaluation will be based on literature research and a summary presentation about the selected topic.

Student should assist  to 80% of all classes.