Chimpanzee homologous of human Y specific STRs - A comparative study and a proposal for nomenclature

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Gusmao, L (Autor) Outra; Gonzalez Neira, A (Autor) Outra; Alves, C (Autor) Outra; Lareu, M (Autor) Outra; Costa, S (Autor) Outra; Amorim, A (Autor) FCUP; Carracedo, A (Autor) Outra

Revista

Título: Forensic Science International

Vol. 126

Páginas: 129-136

ISSN: 0379-0738

Editora: Elsevier

Indexação

ISI Web of Knowledge - 47 Citações

Scopus - 55 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-000-PP6

DOI: 10.1016/s0379-0738(02)00046-4

Abstract (EN): Eleven Y specific microsatellites, previously studied in humans, were typed for fragment length and sequenced in chimpanzees (Pan troglodytes). The primers described by Ayub et al. (Nucleic Acids Res. 28, 2000, 2) for amplifying DYS434, DYS435, DYS436, DYS437, DYS438, DYS439 and those described by White et al. (Genomics, 57,1999,433) for GATA A10, A7.1, A7.2, C4, and H4, were used to amplify DNA samples from chimpanzees. Primers described for Y GATA A4 were found to amplify the same region as reported for DYS439. Moreover, the GATA A4 forward primer only matches the repeat flanking region in 14 of the 28 bp, being responsible for a very weak amplification. Therefore, this system was not included in this study. The analysis of the repeat and sequence structure observed in chimpanzee and human Y chromosomes allowed evolutionary comparisons as well as the basis for improving Y STR nomenclature and therefore, a unified nomenclature for these novel STRs is proposed to the scientific community following ISFG recommendations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8