Genetic diversity of Y-specific STRs in chimpanzees (Pan troglodytes)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Gusmao, L (Autor) Outra; Gonzalez Neira, A (Autor) Outra; Alves, C (Autor) Outra; Sanchez Diz, P (Autor) Outra; Dauber, EM (Autor) Outra; Amorim, A (Autor) FCUP; Carracedo, A (Autor) Outra

Revista

Título: American Journal of Primatology

Vol. 57

Páginas: 21-29

ISSN: 0275-2565

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 10 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-PCQ

DOI: 10.1002/ajp.1084

Abstract (EN): Using the primers described for humans, sequences for 11 Y-specific microsatellites (DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, GATA A10, A7.1, A7.2, C4, and H4 [Gusmao et al., in press]), previously described in 10 male chimpanzees (Pan troglodytes), were confirmed in nine additional male chimpanzees. Sequences for nine additional microsatellites (DYS19, DYS385I and H, DYS389I and H, DYS390, DYS391, DYS392, and DYS393) were determined in all 19 male chimpanzees; homology to human Y-Short Tandem Repeat (STRs) was confirmed by sequencing. Good amplification results were not obtained for DYS19 and DYS385I/II. Two amplicons were obtained for DYS389I/II, but in contrast to humans, the larger fragment was not Y-specific. Moreover, no polymorphism was observed for DYS434, DYS435, or GATA A10. Consequently, these eight STRs were eliminated from further analyses, and haplotype and allele frequencies were estimated for the remaining 12 STRs. A high haplotype diversity value was found (1.000 +/- 0.017), demonstrating the usefulness and informative power of these Y-STRs for future studies on chimpanzee population genetics. (C) 2002 Wiley-Liss, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9