Telomerase activity and genetic alterations in primary breast carcinomas

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Papadopoulou, A (Autor) Outra; Trangas, T (Autor) Outra; Manuel R Teixeira (Autor) ICBAS; Heim, S (Autor) Outra; Dimitriadis, E (Autor) Outra; Tsarouha, H (Autor) Outra; Andersen, JA (Autor) Outra; Evangelou, E (Autor) Outra; Ioannidis, P (Autor) Outra; Agnantis, NJ (Autor) Outra; Pandis, N (Autor) Outra

Revista

Título: Neoplasia

Vol. 5

Páginas: 170-178

ISSN: 1522-8002

Editora: Elsevier

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Outras Informações

ID Authenticus: P-000-HMS

DOI: 10.1016/s1476-5586(03)80009-x

Abstract (EN): It has been proposed that the structural and numerical chromosome abnormalities recorded in breast cancer could be the result of telomere dysfunction and that telomerase is activated de novo to provide a survival mechanism curtailing further chromosomal aberrations. However, recent in vivo and in vitro data show that the ectopic expression of telomerase promotes tumorigenesis via a telomere length-independent mechanism. In this study, the relation between telomerase expression and the extent of chromosomal aberrations was investigated in 62 primary breast carcinomas. Telomerase activity was measured using a polymerase chain reaction-based telomeric repeat amplification protocol assay and 92% of the tumors were found to express telomerase with a relative activity ranging from 0 to 3839.6. Genetic alterations were determined by G-banding and comparative genomic hybridization analysis and 97% of the tumors exhibited chromosomal aberrations ranging from 0 to 44 (average: 10.98). In the overall series, the relationship between telomerase activity levels and genetic changes could be best described by a quadratic model, whereas in tumors with below-average genetic alteration numbers, a significant positive association was recorded between the two variables (coefficient=0.374, P=.017). The relationship between telomerase activity levels and the extent of genetic alteration may reflect the complex effect of telomerase activation upon tumor progression in breast carcinomas.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9