Molecular and Cellular Biology II

Code:

MIMD010202

Acronym:

BCM II

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2024/2025 - 2S

Active?	Yes
Responsible unit:	Mestrado Integrado
Course/CS Responsible:	Integrated Master in Dental Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMD	93	Official Plan from the academic year 2020/2021	1	-	5	81	135
		NÃO INSCREVER - Plano exclusivo para uso administrativo	1	-	5	81	135

Teaching language

Portuguese
Obs.: Português e inglês Obs.: As aulas e os exames serão dadas em Português OU Inglês, dependendo dos estudantes.

Objectives

- Acquisition of knowledge related to the composition, structure, function and metabolism of nucleic acids;



- Acquisition of theoretical skills on molecular mechanisms related to the maintenance, expression and transmission of genetic information;



- Recognition of the molecular complexity underlying all cells;



- Understanding the mechanisms related to the characteristics of different cell types;



- Knowledge of the main molecular biology techniques related to the identification, manipulation and analysis of nucleic acids;



- Recognition of the importance of different molecular biology techniques in the context of health sciences, particularly their importance in terms of molecular diagnosis;



- Recognition of the importance of molecular biology in scientific research.

Learning outcomes and competences

From the theoretical part of the course, students are expected to understand the molecular mechanisms involved in the genetic and biological organization necessary for the correct establishment of the identity of organisms.



On the theoretical-practical part, students are expected to acquire knowledge about Molecular Biology and Recombinant DNA techniques, and their applicability in Dentistry.



From the practical part, students are expected to acquire knowledge of molecular biology techniques, which allow them to purify genomic and plasmid DNA, analyze it, manipulate it and introduce plasmid DNA into bacteria, and design primers for PCR and execute it.

Working method

Presencial

Program

Theoretical themes:



-Nucleic acids: general characteristics



-Chromatin organization



-Replication



-Repair



-Transcription



- Processing of RNAs



-The genetic code and translation



-DNA mutation and repair



-DNA recombination



-Mechanisms for regulating gene expression in prokaryotes and eukaryotes.



-Virus



-Oncogenes and tumor suppressor genes




Theoretical-practical themes:



- Recombinant DNA techniques.



 



Practical topics:



- Identification techniques, manipulation and analysis of nucleic acids

Mandatory literature

Carlos José Correia de Azevedo; Biologia celular e molecular. ISBN: 978-972-757-692-0
Bruce Alberts et al.; Molecular Biology of the Cell, Garland Science, 2008. ISBN: 0-8153-4110-5
Sambrook J, Russell D ; Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001. ISBN: 13.978-0879695774

Teaching methods and learning activities

Theoretical classes explaining different themes, using dynamic and permanent student participation. 

Interactive theoretical-practical classes, with an applied perspective on different subjects related to the theoretical contents of the curricular unit, namely Recombinant DNA techniques. Studnets presentation of a scientific paper.

Practical classes aimed at developing basic skills in Molecular Biology.

Evaluation Type

Evaluation with final exam

Assessment Components

Designation	Weight (%)
Exame	90,00
Apresentação/discussão de um trabalho científico	10,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Estudo autónomo	60,50
Apresentação/discussão de um trabalho científico	5,00
Frequência das aulas	70,00
Total:	135,50

Eligibility for exams

Attendance to 3/4 of all the practical classes and presentation of a paper.

It is ompulsry to bring and use a laboratory coat in the pratical classes.

Students who attended the practical classes in the two previous academic years may eventually be exempt from attending practical classes in the current academic year. However, they must contact the responsible teacher to confirm the exemption.

Calculation formula of final grade

TThe final classification will be 90% final exam and 10% ofthe paper presntation.

The  written final exam will include both the theoretical, teoretical-pratical and the practical parts. 
The exam will have 80% questions related to the theoretical and theoretical part, and 20% related to the practical part.

These components will be assessed in the final exam in which the questions are distributed according to the indicated assessment percentages.

Special assessment (TE, DA, ...)

Students who are exempt from attending practical classes due to student-worker status, or any other status provided by law, must contact the director at the beginning of the semester.