Molecular and Cellular Biology II

Code:

MIMD010202

Acronym:

BCM II

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2023/2024 - 2S

Active?	Yes
Responsible unit:	Mestrado Integrado
Course/CS Responsible:	Integrated Master in Dental Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMD	82	Official Plan from the academic year 2020/2021	1	-	5	81	135
		NÃO INSCREVER - Plano exclusivo para uso administrativo	1	-	5	81	135

Teaching language

Portuguese

Objectives

- Acquisition of knowledge related to the composition, structure, function and metabolism of nucleic acids;



- Acquisition of theoretical skills on molecular mechanisms related to the maintenance, expression and transmission of genetic information;



- Recognition of the molecular complexity underlying all cells;



- Understanding the mechanisms related to the characteristics of different cell types;



- Knowledge of the main molecular biology techniques related to the identification, manipulation and analysis of nucleic acids;



- Recognition of the importance of different molecular biology techniques in the context of health sciences, particularly their importance in terms of molecular diagnosis;



- Recognition of the importance of molecular biology in scientific research.

Learning outcomes and competences

- Acquisition of knowledge related to the composition, structure, function and metabolism of nucleic acids;



- Acquisition of theoretical and theoretical-practical skills on molecular mechanisms related to the maintenance, expression and transmission of genetic information;



- Recognition of the molecular complexity underlying all cells;



- Understanding the mechanisms related to the characteristics of different cell types;



- Knowledge of the main molecular biology techniques related to the identification, manipulation and analysis of nucleic acids and recombinant DNA;



- Recognition of the importance of different molecular biology and recombinant DNA techniques in the context of health sciences, particularly their importance in terms of molecular diagnosis;



- Recognition of the importance of molecular biology in scientific research.

Working method

Presencial

Program

Theoretical themes:



-Nucleic acids: general characteristics



-Chromatin organization



-Replication



-Repair



-Transcription



- Processing of RNAs



-The genetic code and translation



-DNA mutation and repair



-DNA recombination



-Mechanisms for regulating gene expression in prokaryotes and eukaryotes.



-Virus



-Oncogenes and tumor suppressor genes



Theoretical-practical themes:



- Recombinant DNA techniques.



 



Practical topics:



- Identification techniques, manipulation and analysis of nucleic acids

Mandatory literature

Carlos José Correia de Azevedo; Biologia celular e molecular. ISBN: 978-972-757-692-0
Bruce Alberts et al.; Molecular Biology of the Cell, Garland Science, 2008. ISBN: 0-8153-4110-5
Sambrook J, Russell D ; Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001. ISBN: 13.978-0879695774

Teaching methods and learning activities

Theoretical classes explaining different themes, using dynamic and permanent student participation. Interactive theoretical-practical classes, with an applied perspective on different subjects related to the theoretical contents of the curricular unit, namely Recombinant DNA techniques. Practical classes aimed at developing basic skills in Molecular Biology.

Evaluation Type

Evaluation with final exam

Assessment Components

Designation	Weight (%)
Exame	100,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Estudo autónomo	54,00
Total:	54,00

Eligibility for exams

Attendance at 3/4 of theoretical-practical classes and 3/4 of practical classes.

Calculation formula of final grade

The final classification consists of the classification obtained in the final exam.

In the final exam, 80% relates to the content taught in theoretical and theoretical-practical classes, and 20% relates to the content taught in practical classes. These components will be assessed in the final exam in which the questions are distributed according to the indicated assessment percentages.

Special assessment (TE, DA, ...)

Students who are exempt from attending practical classes due to student-worker status,
or any other status provided by law, must contact the director at the beginning of the semester.