Molecular and Cell Biology II

Code:

MD1203

Acronym:

BCM II

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2013/2014 - 2S

Active?	Yes
Responsible unit:	Mestrado Integrado
Course/CS Responsible:	Integrated Master in Dental Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMD	97	PLANO OFICIAL 2007/2008 BOLONHA	1	-	6	90	162

Teaching language

Portuguese

Objectives

In this course we hope to provide the student with a solid knowledge about the cell molecular biology and organization, while encouraging literature research, discussion and collaboration between students in their learning process.

Learning outcomes and competences

The student must acquire theoretical knowledge in the following areas: - Cell membrane Structure - Sorting and intracellular protein traffic - Cell Communication - Cytoskeleton and extracellular matrix - Cell cycle and programmed cell death - Mechanisms of cell division - Developmental biology The student must develop laboratory work skills , as well as the preparation of scientific reports.

Working method

Presencial

Program

Membrane structure. The Lipid Bilayer and membrane proteins. Membrane transport. The Electrical properties of membranes. The nucleus. Transport of molecules between the nucleus and the cytosol. Protein transport to the mitochondria. Peroxisomes and ER. Intracellular vesicular traffic. Transport from the ER though the Golgi apparatus. Transport to the lysosomes. Endocytosis and Exocytosis. The Cytoskeleton. Cellular shape and mobility Molecular motors. Extracellular matrix. General principles of cell communication. Cell signalling. Cell cycle and mitosis. Cell proliferation control, apoptosis and cancer. Meiosis and fertilization. Developmental Biology:Biological models to study developmental biology. Segmentation and gastrulation in Xenopus Laevis. Genetic regulation and embryonic axis formation in Drosophila melanogaster.

Practical applications of DNA technology:Western, Southern and Northern Blotting; DNA sequencing, DNA fingerprinting, PCR analysis of human DNA, Molecular diagnosis of human disease,  Nucleus and chromatin organelles and cellular components. Cell cycle analysis. Meiosis and germ cells. Developmental biology: drosophila.

Complementary Learning: Science case studies and preparation of an integrated seminar (with Biochemistry II and Epidemiology, Biostatistics II and Communication and Information technology) to present in the end of the semester.

Case Studies:

Case study 1 :Agony and Ecstasy - A Case Study on Cell Membrane Structure and Function by Norris Armstrong, Biology Department, University of Georgia.

Case study 2: Colon cancer A Case of Genetic Bad Luck? by Anne M. Casper, Department of Molecular Genetics and Microbiology Duke University.

Scientific Seminar: Students (in groups of 4) prepare a seminar integrated with Biochemistry II, Epidemiology / Biostatistics II and Technologies of Information and Communication, for presentation at the end of the school year. Scientific subjects addressed in the seminars and then discussed with  professors and colleagues.

Mandatory literature

Alberts Bruce 070; Essential Cell Biology. ISBN: 978-0-8153-4129-1
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter; Molecular Biology of the Cell, 5th edition, Garland Science, 2007. ISBN: 978-0-8153-4106-2
Cooper Geoffrey M.; The^cell. ISBN: 0-87893-220-7

Complementary Bibliography

Steven R Goodman; Medical Cell Biology, Academic Press, 2008
Lodish Harvey 070; Molecular cell biology. ISBN: 0-7167-4366-3
Alberts Bruce 070; Molecular biology of the cell. ISBN: 0-8153-4072-9
Carlos Azevedo/Claudio E. Sunkel; Biologia Celular e Molecular, 5ª Edição, Lidel, 2012

Teaching methods and learning activities

Active learning: case studies, problem-based learning and paper discussions.

Laboratory practical classes including lab experiments, writting of scientific reports and problem solving.

Software

Moodle

keywords

Natural sciences > Biological sciences > Biology > Human biology
Natural sciences > Biological sciences > Biology > Cell biology
Natural sciences > Biological sciences > Biology > Molecular biology
Natural sciences > Biological sciences > Biology > Clinical biology

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
	Participação presencial		10,00
	Prova oral		15,00
	Teste		45,00
	Trabalho laboratorial		30,00
	Total:	-	100,00

Eligibility for exams

Students must attend at least 75% of all classes to be admitted to exams. Students who fail their exams are not obliged to attend classes the following year.

Calculation formula of final grade

Theory (T) and Continuous evaluation (C) : laboratory and seminar presentation Theory: 1 test (two hours) Continuous evaluation: Laboratory work and reports, problem resolution and Integrative seminar presentation.

The final mark is calculated as follows: Theory test (T) (maximum score 9/20) + Laboratory (maximum score 6/20) + Seminar presentation (maximum score 3/20) + class attendance (maximum score 2/20).

The student needs to score at least 9/20 as a minimal grade in each component (Theory and Continuous evaluation).

Students who fail continuous assessment may request a final global exam Gobal exam final mark* : Theory exam (maximum score 12/20) + Lab Exam (maximum score 6/20) + class attendance (maximum score 2/20) The student must attend at least 75% of all classes and have a final mark of at least 10/20 to pass the course.

* See Comments

Special assessment (TE, DA, ...)

These students will be alowed to have alternative exam dates, in accordance with the law. Final mark is obtained as follows: Theory exam (maximum score 13/20) + Lab Exam (maximum score 7/20).

Classification improvement

It is obligatory to evaluate theory and laboratory components of the discipline. Final mark is obtained as follows: Theory exam (maximum score 3/20) + Lab Exam (maximum score 7/20)

Observations

Students who failed the theory test but scored 14/20 or higher in their Continuous evaluation (Laboratory + Seminar presentation) are excused from the Lab exam. In this case final mark is calculated as follows: Theory exam (maximum score 9/20) + Continuous evaluation (maximum score 9/20) + class attendance (maximum score 2/20).

Hours for Students:

Mondays 13:00-17:00h

Tuesdays 9:00-12:00h

Fridays 9:00-12:00h.