GLUTATHIONE AND CYSTEINE MEASUREMENT IN BIOLOGICAL SAMPLES BY HPLC WITH A GLASSY-CARBON WORKING DETECTOR

• Type: Article in International Scientific Journal
• Date: 1994

Authors

CARVALHO, FD (Author) FFUP; REMIAO, F (Author) Other; VALE, P (Author) Other; TIMBRELL, JA (Author) Other; BASTOS, ML (Author) FFUP; FERREIRA, MA (Author) Other

Journal

Title: Biomedical Chromatography

Vol. 8

Pages: 134-136

ISSN: 0269-3879

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 48 Citations

Scopus - 47 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-K2V

DOI: 10.1002/bmc.1130080308

Abstract (EN): An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 +/- 4.15, 4.50 +/- 0.65, and 0.19 +/- 0.12 mM (means +/- SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N-ethylmaleimide.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3