Oxidation process of adrenaline in freshly isolated rat cardiomyocytes: Formation of adrenochrome, quinoproteins, and GSH adduct

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Vera Marisa Costa (Author) Other; Renata Silva (Author) Other; Luisa Maria Ferreira (Author) Other; Paula Serio Branco (Author) Other; Felix Carvalho (Author) FFUP; Maria Lourdes Bastos (Author) FFUP; Rui Albuquerque Carvalho (Author) Other; Marcia Carvalho (Author) Other; Fernando Remiao (Author) FFUP

Journal

Title: Chemical Research in Toxicology

Vol. 20

Pages: 1183-1191

ISSN: 0893-228X

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 47 Citations

Scopus - 58 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-89X

DOI: 10.1021/tx7000916

Abstract (EN): High concentrations of circulating biogenic catecholamines often exist during the course of several cardiovascular disorders. Additionally, coronary dysfunctions are prominent and frequently related to the ischemic and reperfusion phenomenon (I/R) in the heart, which leads to the release of large amounts of catecholamines, namely adrenaline, and to a sustained generation of reactive oxygen species (ROS). Thus, this work aimed to study the toxicity of adrenaline either alone or in the presence of a system capable of generating ROS [xanthine with xanthine oxidase (X/XO)], in freshly isolated, calcium tolerant cardiomyocytes from adult rats. Studies were performed for 3 h, and cardiomyocyte viability, ATP level, lipid peroxidation, protein carbonylation content, and glutathione status were evaluated, in addition to the formation of adrenalines oxidation products and quinoproteins. Intracellular GSH levels were time-dependently depleted with no GSSG formation when cardiomyocytes were exposed to adrenaline or to adrenaline with X/XO. Meanwhile, a time-dependent increase in the rate of formation of adrenochrome and quinoproteins was observed. Additionally, as a new outcome, 5-(glutathion- S-yl)adrenaline, an adrenaline adduct of glutathione, was identified and quantified. Noteworthy is the fact that the exposure to adrenaline alone promotes a higher rate of formation of quinoproteins and glutathione adduct, while adrenochrome formation is favored where ROS production is stimulated. This study shows that the redox status of the surrounding environment greatly influences adrenalines oxidation pathway, which may trigger cellular changes responsible for cardiotoxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9