Natriuretic peptide system modulation in uninephrectomized rats

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Santos Araujo, C (Author) Other; Roncon Albuquerque, R (Author) Other; Moreira Rodrigues, M (Author) ICBAS; Henriques Coelho, T (Author) Other; Quelhas Santos, J (Author) FMUP; Faria, B (Author) Other; Benedita Sampaio-Maia (Author) FMDUP; Leite-Moreira AF (Author) FMUP; Pestana M (Author) FMUP

Journal

Title: Experimental and Clinical Cardiology

Vol. 20 No. 8

Pages: 2368-2387

ISSN: 1205-6626

Publisher: CARDIOLOGY ACADEMIC PRESS

Indexing

Scopus - 1 Citation

Scientific classification

FOS: Medical and Health sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-009-V98

Abstract (EN): BACKGROUND: Cardiac activation of the natriuretic peptide (NP) system plays a major role in the renal adaptations to volume, expansion (VE). However, its role after uninephrectomy (Unx) remains to be elucidated. In the present study, we evaluated cardiac hemodynamics, plasma NP-Type B (BNP) levels nd NP receptor A (NPR-A) and C (NPR-C) expression in the renal cortex (RC) and medulla (RM) of Sham and Unx rats. METHODS: Male Wistar-Han rats (n=42) were randomly assigned to Unx or Sham surgery. Non-invasive blood pressure (BP), invasive left ventricular (LV) hemodynamics and natriuretic response to VE were performed and plasma was collected for BNP determination, 2, 10 and 26 weeks after surgery. In addition, samples from RC and RM were obtained for NPR-A and -C quantification (RT-PCR and western-blot). RESULTS: In Unx, blood pressure elevation and reduced natriuretic response to VE were observed. Although plasma BNP levels increased overtime, no LV dysfunction or myocardial BNP gene activation was observed in Unx. NPR-A mRNA in RC and RM were significantly increased in Unx at 26 weeks. This was accompanied by decreased NPR-C mRNA in the RM in Unx at 10 and 26 weeks, with no differences detected in the RC. CONCLUSIONS: In our experimentalmodel of Unx, progressive BP elevation and reduced natriuretic response to VE were accompanied by modulation of NPR-A and -C expression, in both the RC and RM, in the absence of cardiac activation of the NP system. Our results suggest a local renal modulation of the NP system after Unx. © 2013 et al.; licensee Cardiology Academic Press.

Language: English

Type (Professor's evaluation): Scientific