Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Patricia Ferreira (Author) Other; Teresa Cardoso (Author) Other; Filipa Ferreira (Author) Other; Manuel Fernandes Ferreira (Author) FCUP; Peter Piper (Author) Other; Maria Joao Sousa (Author) Other

Journal

Title: FEMS Yeast Research

Vol. 14

Pages: 1006-1014

ISSN: 1567-1356

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 35 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Natural sciences

Other information

Authenticus ID: P-00A-07R

DOI: 10.1111/1567-1364.12189

Abstract (EN): Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9