The Benefit of Bone Marrow Concentrate in Addition to a Glass-Reinforced Hydroxyapatite for Bone Regeneration: An In Vivo Ovine Study

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Joao Torres (Author) Other; Manuel Gutierres (Author) Other; Luis Atayde (Author) Other; Paulo Cortez (Author) Other; Maria Ascensão Lopes (Author) FEUP; Santos, JD (Author) FEUP; A. T. Cabral (Author) Other; Carola F. van Eck (Author) Other

Journal

Title: Journal of Orthopaedic Research

Vol. 35

Pages: 1176-1182

ISSN: 0736-0266

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Scientific classification

FOS: Engineering and technology > Medical engineering

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-00P-JEY

DOI: 10.1002/jor.22800

Abstract (EN): This study evaluates the ability of a Glass Reinforced Hydroxyapatite Composite (GRHC), in a new microporous pellet formulation with autologous bone marrow concentrate (BMC), to enhance bone regeneration and new bone formation. Ninety non-critical sized bone defects were created in the femurs of nine Merino breed sheep and randomly left unfilled (group A), filled with GRHC pellets alone (group B) or filled with GRHC pellets combined with BMC (group C). The sheep were sacrificed at 3 weeks (three sheep), 6 weeks (three sheep) and 12 weeks (three sheep) and histological analysis (Light Microscopy-LM), scanning electron microscopy (SEM) and histomorphometric analysis (HM) were performed. At 3, 6, and 12 weeks, HM revealed an average percentage of new bone of 48, 72, 83%; 25, 73, 80%, and 16, 38, 78% for Groups C, B and A respectively (significantly different only at 3 weeks p<0.05). LM and SEM evaluation revealed earlier formation of well-organized mature lamellar bone in Group C. This study demonstrates that the addition of a bone marrow concentrate to a glass reinforced hydroxyapatite composite in a pellet formulation promotes early bone healing. (C) 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7