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Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system

Title
Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system
Type
Article in International Scientific Journal
Year
2012
Authors
Clara Pereira
(Author)
Other
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Edite Lopes
(Author)
Other
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Joao R Mesquita
(Author)
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Sao Jose Nascimento, MSJ
(Author)
FFUP
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Lucilia Saraiva
(Author)
FFUP
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Journal
Vol. 91
Pages: 358-360
ISSN: 0167-7012
Publisher: Elsevier
Scientific classification
FOS: Natural sciences > Biological sciences
Other information
Authenticus ID: P-002-2K3
Abstract (EN): The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.
Language: English
Type (Professor's evaluation): Scientific
Contact: clarafrancisco@gmail.com; lucilia.saraiva@ff.up.pt
No. of pages: 3
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