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Cross-species genetic markers: a useful tool to study the world's most threatened wild equid-Equus africanus

Title
Cross-species genetic markers: a useful tool to study the world's most threatened wild equid-Equus africanus
Type
Article in International Scientific Journal
Year
2012
Authors
rosenbom, s
(Author)
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costa, v
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steck, b
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moehlman, p
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beja-pereira, a
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FCUP
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Journal
Vol. 58
Pages: 609-613
ISSN: 1612-4642
Publisher: Springer Nature
Scientific classification
FOS: Natural sciences > Biological sciences
Other information
Authenticus ID: P-002-9MR
Abstract (EN): Once a diverse family, the Equidae family is now reduced to a single genus, Equus. From the seven extant species of the genus, the African wild ass (Equus africanus) is the most threatened with extinction (last survey indicated 600 individuals). In this work we tested 25 published microsatellite primer pairs isolated from the horse genome on 22 African wild ass (E. africanus) individuals from wildlife reserves and zoos. From the 25 loci tested, 15 amplified well and showed moderate allelic richness (5.06, mean number of alleles) and moderately high expected heterozigosity (0.59). Although all possible loci pairs showed no significant gametic disequilibrium (P > 0.007), deviations from Hardy-Weinberg proportions were found in 2 out of the 15 analysed microsatellite loci (AHT5 and VHL20). Here, we propose these polymorphic markers to be used as a standard set in future studies on population and conservation genetics of the African wild ass.
Language: English
Type (Professor's evaluation): Scientific
Contact: albanobp@fc.up.pt
No. of pages: 5
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