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Carbon dots from tryptophan doped glucose for peroxynitrite sensing

Title
Carbon dots from tryptophan doped glucose for peroxynitrite sensing
Type
Article in International Scientific Journal
Year
2014
Authors
simoes, efc
(Author)
Other
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da silva, jcge
(Author)
FCUP
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leitao, jmm
(Author)
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Journal
Vol. 852
Pages: 174-180
ISSN: 0003-2670
Publisher: Elsevier
Scientific classification
FOS: Natural sciences > Chemical sciences
Other information
Authenticus ID: P-009-YCQ
Abstract (EN): Tryptophan doped carbon dots (Trp-CD) were microwave synthesized. The optimum conditions of synthesizing of the Trp-CD were established by response surface multivariate optimization methodologies and were the following: 2.5 g of glucose and 300 mg of tryptophan diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700 W. Trp-CD have an average size of 20 nm, were fluorescent with a quantum yield of 12.4% and the presence of peroxynitrite anion (ONOO ) provokes quenching of the fluorescence. The evaluated analytical methodology for ONOO detection shows a linear response range from 5 to 25 mu M with a limit of detection of 1.5 mu M and quantification of 4.9 mu M. The capability of the ONOO quantification was evaluated in standard solutions and in fortified serum samples.
Language: English
Type (Professor's evaluation): Scientific
Contact: jleitao@ff.uc.pt
No. of pages: 7
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