STARFISH ACROSOMAL ACID-PHOSPHATASE - A CYTOCHEMICAL AND BIOCHEMICAL-STUDY

• Type: Another Publication in an International Scientific Journal
• Date: 1988

Authors

SOUSA, M (Author) ICBAS; FERREIRA, PM (Author) ICBAS; AMORIM, A (Author) FCUP; AZEVEDO, C (Author) Other

Journal

Title: Biology of the Cell

Vol. 63

Pages: 101-104

ISSN: 0248-4900

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-TM5

DOI: 10.1016/0248-4900(88)90113-x

Abstract (EN): The acrosome reaction of spermatozoa from the starfish Marthasterias glacialis was induced with the ionophore A23187. Reacted cells were then processed for acid phosphatase ultrastructural cytochemistry, but significant enzyme activity was not detected. However, when the supernates from suspensions of ionophore-treated sperm were assayed for acid phosphatase, a net enzyme activity was observed. Supernatant proteins were run in starch gel electrophoresis and fluorescent zymograms revealed a single band of acid phosphatase. SDS-PAGE of proteins eluted from the active spots of starch gels showed one major band of about 63 kDa. The results obtained support the hypothesis that the acid phosphatase whose activity has been detected only at the time of binding of sperm and egg originates from the sperm acrosome. © 1988.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4