SUBTYPING OF ALKYLATED HUMAN OROSOMUCOID - EVIDENCE FOR A DUPLICATED GENE, ORM1-ASTERISK-F2S

• Type: Article in International Scientific Journal
• Date: 1993

Authors

ROCHA, J (Author) FCUP; AMORIM, A (Author) FCUP; LUCKENBACH, C (Author) Other; KOMPF, J (Author) Other; RITTER, H (Author) Other

Journal

Title: Electrophoresis

Vol. 14

Pages: 235-237

ISSN: 0173-0835

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-N9V

DOI: 10.1002/elps.1150140138

Abstract (EN): Isoelectric focusing of human orosomucoid (ORM) was studied following different sample treatment. It is shown that: (i) alkylation with iodoacetamide leads to a drastic change in the isoelectric point (pI) of both ORM1 F2 and ORM2 A gene products and greatly improves the discrimination between ORM1 F1 and ORM1 F2; (ii) previous reduction of the molecule with dithiothreitol partially inhibits the pI transitions with resultant artifactual ORM1 F1F2S patterns that correspond in most cases to F2S phenotypes. With the technique now described, the persistence of three ORM1 gene products was found in only one individual and the segregation analysis is consistent with the existence of a rare ORM1*F2S haplotype.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3