Detection of cylindrospermopsin toxin markers in cyanobacterial algal blooms using analytical pyrolysis (Py-GC/MS) and thermally-assisted hydrolysis and methylation (TCh-GC/MS)

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ríos, V (Author) Other; Ana I Prieto (Author) Other; Ana M Cameán (Author) Other; F.J González-Vila (Author) Other; J.M de la Rosa (Author) Other; Vitor Vasconcelos (Author) Other; J.A González-Pérez (Author) Other

Journal

Title: Chemosphere

Vol. 108

Pages: 175-182

ISSN: 0045-6535

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-009-GWF

DOI: 10.1016/j.chemosphere.2014.01.033

Abstract (EN): The hepatotoxin cylindrospermopsin (CYN) is produced by freshwater cyanobacteria becoming an emerging threat for human health. Methods for the rapid determination of CYN in environmental samples are needed. Conventional analytical pyrolysis (Py-GC/MS) and thermally-assisted hydrolysis and methylation (TCh-GC/MS) were used to study a CYN standard, two Aphanizomenon ovalisporum cultures (CYN+) and one culture of Cylindrospermopsis raciborskii (CYN-). A micro-furnace pyrolyzer was used directly attached to a GC/MS system fitted with a 30 m x 250 mu m x 0.25 mu m film thickness column (14% cyanopropyl phenyl, 86% dimethyl polysiloxane pahase composition). Oven temperature was held at 50 degrees C for 1 min and increased to 100 degrees C at 30 degrees C min(-1), from 100 degrees C to 300 degrees C at 10 degrees C min(-1), and stabilized at 300 degrees C for 10 min using helium (1 mL min(-1)) as carrier gas. Pyrolysis at 500 degrees C yield over 70 compounds with 20 specific for CYN+ samples. Two peaks containing a diagnostic fragment (m/z 194) were found at 25.0 and 28.9 min only in CYN+ samples. Fewer peaks with limited diagnostic value were released after TCh-GC/MS, including breakdown products and TMAH adducts. A compound was detected that may correspond to the CYN molecule (MW 415 Da) thermoevaporation product after the loss of SO3 (MW 80 Da). This TCh-GC/MS peak (m/z 336) together with the fragments obtained by conventional Py-GC/MS (m/z 194) are diagnostic ions with potential use for the direct detection of CYN toxin in environmental samples at last with an estimated 5 ppm detection threshold.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8